DNA原位杂交和免疫组化染色检测HPV的比较  

Comparison of DNA in situ hybridization and immunohistochemical staining method in the detection of HPV

作　　者：禤瑞霞[1] XUAN Ruixia(Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,China)

机构地区：[1]中山市中医院,广东中山528400

出　　处：《包头医学院学报》2018年第11期64-65,共2页Journal of Baotou Medical College

摘　　要：目的:探讨DNA原位杂交和免疫组化染色检测人乳头瘤病毒(human papilloma virus,HPV)的效果。方法:选择103例外阴尖锐湿疣患者,分别采用DNA原位杂交、免疫组化染色方法进行检测,比较分析两种检测结果。结果:DNA原位杂交的阳性检测率为93. 20%,高于免疫组化阳性检测率(66. 02%)(P <0. 05)。结论:DNA原位杂交检测技术用于尖锐湿疣患者中具有较高阳性率,可作为尖锐湿疣诊断及治疗的可靠依据。Objective:To investigate the effect of DNA in situ hybridization and immunohistochemical staining method in the detection of human papilloma virus(HPV).Methods:103 patients with condyloma acuminatum were selected and detected by DNA in situ hybridization and immunohistochemical staining respectively.The results were compared and analyzed.Results:The positive rate of DNA in situ hybridization was 93.20%,which was significantly higher than that in immunohistochemistry(66.02%),and the difference was statistically significant(P<0.05).Conclusion:DNA in situ hybridization has a high positive rate in patients with condyloma acuminatum,which can be used as a reliable basis for the diagnosis and treatment of condyloma acuminatum.

关 键 词：DNA原位杂交 免疫组化染色 尖锐湿疣 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]