三味檀香散对大鼠脑缺血后不同时相血清中IL-1β、IL-6和IL-8含量的影响  

Effect of Sanwei Tanxiang powder on serum content of IL-1β,IL-6 and IL-8 in cerebral ischemia rats at different time stages

作　　者：何静波 王瑞芳 杨玉梅[1] 张宁[1] 王远霆 胡娟[1] 安萌萌 沈钺 HE Jingbo;WANG Ruifang;YANG Yumei;ZHANG Ning;WANG Yuanting;HU Juan;AN Mengmeng;SHEN Yue(Department of Pharmacy,Baotou Medical College,Baotou 014060,China;Grade 2017 undergraduate students of Pharmacy,Baotou Medical College)

机构地区：[1]内蒙古科技大学包头医学院药学院,内蒙古包头014060

出　　处：《包头医学院学报》2018年第11期78-80,共3页Journal of Baotou Medical College

基　　金：内蒙古自然科学基金(2016MS08102,2018MS08051);内蒙古科学技术协会项目(20160306);内蒙古自治区高等学校科学研究项目(NJZY18196);包头市医药卫生基金项目(2016X1030-5-4)

摘　　要：目的:观察蒙药三味檀香散对大鼠脑缺血后不同时相IL-1β、IL-6和IL-8含量的影响,探讨其治疗脑缺血损伤可能的作用机制。方法:160只雄性SD大鼠随机分为脑缺血模型组、假手术组、三味檀香散大(1. 5 g/kg)、小剂量组(0. 5 g/kg),每组又随机分为4个时相组,即6 h、12 h、24 h、72 h组。按不同分组分别灌胃给药,1次/d,连续21天后,线栓法制备大鼠大脑中动脉栓塞模型,神经功能评分后,酶联免疫吸附法测定血清IL-1β、IL-6和IL-8的含量。结果:三味檀香散显著降低脑缺血大鼠神经功能评分,并在各时相中不同程度降低脑缺血大鼠血清IL-1β、IL-6和IL-8的含量。结论:三味檀香散对大鼠脑缺血有较好的保护作用;其治疗脑缺血损伤机制与降低炎症因子表达水平有关。Objective:To observe the influence of Sanwei Tanxiang powder on content of IL-1β,IL-6 and IL-8 in cerebral ischemia rats at different time stages and explore its possible treatment mechanism of cerebral ischemia injury.Methods:160 male SD rats were randomly divided into cerebral ischemia model group,sham operation group,high dose group(Sanwei Tanxiang powder 1.5 g/kg)and small dose group(Sanwei Tanxiang powder 0.5 g/kg));each group was randomly divided into four groups(6 h,12 h,24 h,72 h group).Each group was given by gavage respectively once a day for 21 days in a row.The cerebral arterial embolism models were prepared by line ligation method.After the evaluation of nerve function,serum IL-1β,IL-6 and IL-8 levels were determined by ELISA(enzyme linked immunosorbent assay)method.Results:Sanwei Tanxiang powder significantly reduces the nerve function score in cerebral ischemia rats and decreases the serum IL-1β,IL-6 and IL-8 levels at different time stages.Conclusion:Sanwei Tanxiang powder has better protective effect on cerebral ischemia rats.The treatment mechanism of brain ischemia injury is associated with reduced inflammatory factor expression level.

关 键 词：三味檀香散 脑缺血 IL-1Β IL-6 IL-8 

分 类 号：R285.5[医药卫生—中药学]