构建金纳米粒子标记的凝集素芯片应用于抗生素与金黄色葡萄球菌相互作用研究  

Fabrication of Lectin Microarray for Studying Interactions of Antibiotics with Staphylococcus aureus by Gold Nanoparticle Probes

作　　者：李铸衡[1,2] 高晶清[2] 简明红 王振新[2] LI Zhu-Heng;GAO Jing-Qing;JIAN Ming-Hong;WANG Zhen-Xin(Jilin Provincial Institute of Education,Changchun 130024,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]吉林省教育学院,长春130024 [2]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022

出　　处：《分析化学》2018年第12期1904-1912,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21475126)资助~~

摘　　要：建立了一种基于凝集素芯片的共振光散射(Resonance light scattering,RLS)法,用于研究金黄色葡萄球菌(Staphylococcus aureus,S. aureus)与3种广谱抗生素(阿莫西林、万古霉素和链霉素)间的相互作用。本方法通过固定于基片表面的凝集素捕获细菌,使用西非单叶豆凝集素II(Griffonia simplicifolia II,GSⅡ)修饰的38.5 nm金纳米粒子(GNP@GSⅡ)标记捕获的S. aureus获取RLS信号。考察了抗生素存在下S. aureus与16种凝集素亲和能力的变化,发现阿莫西林和万古霉素能够显著降低S. aureus表面糖基化合物的表达种类和表达量;链霉素则能够提高S. aureus表面糖基化合物的表达水平。另外,根据凝集素与S. aureus的特异性识别图谱以及GSⅡ子阵列对S. aureus捕获量变化,能够获得不同抗生素对S. aureus的最小抑制浓度(Minimum inhibitory concentration,MIC)和杀菌效率。The lectin microarray-based resonance light scattering(RLS)assay has been developed to evaluate the interactions of Staphylococcus aureus(S.aureus)with three broad-spectrum antibiotics(amoxicillin,vancomycin and streptomycin).S.aureus was firstly captured by immobilized lectins on the substrate surface,and then labeled with Griffonia simplicifolia II(GSⅡ)modified 38.5 nm gold nanoparticles(GNP@GSⅡ)for generating RLS signal.The investigation of the binding affinity of S.aureus with 16 lectins in the presence of antibiotics showed that amoxicillin and vancomycin could significantly reduce the expression level of glycans on S.aureus membrane,while streptomycin could increase the expression level of glycans on S.aureus membrane.The experimental result may suggest the different action mechanisms of antibiotics with S.aureus.Furthermore,the minimum inhibitory concentration(MIC)values and bactericidal efficiency of antibiotics could also be determined by the drug induced change of RLS intensity of GSⅡsubarray and lectin binding patterns of S.aureus,respectively.

关 键 词：凝集素芯片 金黄色葡萄球菌 抗生素 金纳米粒子 共振光散射 

分 类 号：TQ465[化学工程—制药化工]