柯里拉京对顺铂抗卵巢癌Hey和SKOV3细胞的增敏作用  

Sensitization Effect of Corilagin on Cisplatin Against Ovarian Cancer Hey and SKOV3 Cells

作　　者：李旭丹[1,2] 林志灿[2] 李煊 郑志忠[2] 明艳林[1,2] LI Xudan;LIN Zhican;LI Xuan;ZHENG Zhizhong;MING Yanlin(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China;Key Laboratory of Plant Introduction and Quarantine and Plant Source Products of Xiamen, Xiamen Overseas Chinese Subtropical Plant Introduction Park,Xiamen 361002,China)

机构地区：[1]华侨大学化工学院,福建厦门361021 [2]厦门华侨亚热带植物引种园厦门市植物引种检疫与植物源产物重点实验室,福建厦门361002

出　　处：《华侨大学学报（自然科学版）》2018年第6期892-898,共7页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(81274149)

摘　　要：探讨柯里拉京(corilagin)与羟基喜树碱(HCPT)、顺铂(cDDP)单独及联合用药,体外抗卵巢癌细胞Hey,SKOV3的活性及与cDDP联用机制的初步研究.利用四唑盐(MTT)比色法检测柯里拉京,HCPT,cDDP在不同质量浓度下,单独及联合用药对卵巢癌细胞的增殖抑制率,并根据金氏公式计算其协同指数(Q),进而评价两药联用的增敏效果.通过形态学观察吖啶橙(AO)/溴化乙啶(EB)和Hoechst 33258染色及线粒体膜电位变化,探究其诱导机制.结果表明:柯里拉京对卵巢细胞Hey,SKOV3的半抑制浓度(IC_(50)值)分别为19.20,17.17μmol·L^(-1);柯里拉京与cDDP联合作用对于SKOV3,Hey两种细胞均有协同作用,与HCPT联用只对SKOV3细胞有协同作用,对Hey细胞仅是相加效应;联合用药组对卵巢癌细胞的抑制效果显著高于单独用药组;柯里拉京与cDDP联合用药对卵巢癌细胞的杀伤效果可能是通过诱导细胞凋亡实现的.The activity against ovarian cancer cells(Hey,SKOV3)was investigated in vitro using corilagin,hydroxy camptothecine(HCPT),and cisplatin(cDDP)alone or in combination,and the combination mechanism of corilagin with cDDP was preliminarily studied.The inhibitory rate of corilagin,HCPT and cDDP alone or in combination with different concentrations on ovarian cancer cells was also determined by methyl thiazolyl tetrazolium(MTT)method.And the synergistic index(Q)which assesses the sensitization effect of colilagin for HCPT and cDDP was calculated according to Kim′s formula.In addition,morphological observation and apoptosis staining including acridine orange(AO)/ethidium bromide(EB)and Hoechst 33258 and mitochondrial membrane potential staining were used to explore its potential induction mechanism.The results show that the half maximal inhibitory concentration(IC 50 values)of corilagin on the ovarian cells Hey and SKOV3 were 19.20 and 17.17μmol·L-1,respectively.The combination of corilagin and cDDP had synergistic effects on both SKOV3 and Hey cells,while the combination of colilagin and HCPT only had synergistic effect on SKOV3 and additive effect on Hey.The inhibitory effect of combination therapy group on ovarian cancer cells was significantly higher than that of the control group.Moreover,the killing effect of corilagin in combination with cisplatin on ovarian cancer cells may be realized through the induction of cell apoptosis.

关 键 词：柯里拉京 顺铂 卵巢癌 增敏作用 细胞凋亡 四唑盐比色法 

分 类 号：R285[医药卫生—中药学]