大白菜BrCNGC全基因组鉴定及其表达分析  

Genome-wide identification and expression analysis of BrCNGC in Chinese cabbage

作　　者：汪影 张昌伟[1] 吕善武 侯喜林[1] WANG Ying;ZHANG Changwei;Lv Shanwu;HOU Xilin(State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Ministry of Agriculture and Rural Affairs,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/农业农村部华东地区园艺作物生物学与种质创新重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2018年第6期994-1002,共9页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金重点项目(31330067);中央高校基础研究基金项目(KYTZ201401);江苏省蔬菜产业技术体系项目[SXGC(2017)273];国家大宗蔬菜产业技术体系项目(CARS-23-A-06)

摘　　要：[目的]本文旨在研究大白菜环核苷酸门控离子通道基因(BrCNGC)的进化关系、结构特征、染色体定位及其表达模式。[方法]通过生物信息技术对大白菜BrCNGC进行分子进化分析、功能结构分析、染色体定位分析、保守结构域分析,并且通过荧光定量PCR分析该基因家族在脱落酸(ABA)+芜菁花叶病毒(TuMV)、水杨酸(SA)+TuMV、茉莉酸甲酯(MeJA)+TuMV和抗坏血酸(AsA)+TuMV处理下的表达差异。[结果]大白菜BrCNGC家族有26个成员,可分为4个组(Ⅰ、Ⅱ、Ⅲ、Ⅳ),其中第Ⅳ组又可以分为2个亚组(Ⅳa和Ⅳb)。它们分布在9条染色体上,1号染色体上BrCNGC数量最多(有5个),5号染色体上只有1个BrCNGC基因,而8号染色体上没有BrCNGC基因分布。基因结构分析表明:该家族基因中共鉴定出10种motif,其中有14个BrCNGC蛋白都包含这10种motif,同组成员之间的motif组成相似。荧光定量PCR结果表明:在植物生长调节剂与TuMV相互作用下,大部分BrCNGC的表达量上调,少数BrCNGC的表达量下调。[结论]大白菜BrCNGC结构高度保守,其在大白菜抵御TuMV侵染过程中发挥一定的作用,该发现为以后进一步研究BrCNGC的功能奠定了基础。[Objectives]The aim of this study is to investigate the evolutionary relationships,structural features,chromosomal location of the cyclic nucleotide gated ion channel genes in Chinese cabbage(BrCNGC)and to analyze their expression parterns under different treatments.[Methods]The bioinformatics techniques were used to analyze the molecular evolution,genes structure,chromosomal location and conserved motifs of BrCNGC in Chinese cabbage.The expression level of BrCNGC was analyzed by quantitative RT-PCR under different treatments:abscisic acid(ABA)+Turnip mosaic virus(TuMV),salicylic acid(SA)+TuMV,methyl jasmonate(MeJA)+TuMV,and ascorbic acid(AsA)+TuMV.[Results]The results showed that there are 26 BrCNGC genes in Chinese cabbage and can be classified into four major groups(Ⅰ,Ⅱ,Ⅲ,Ⅳ)and two subgroups(Ⅳa andⅣb),they were predicted to be located on 9 chromosomes,the chromosome 1 contained five BrCNGC,while the chromosome 5 only contained one BrCNGC and no BrCNGC on chromosome 8.Gene structure analysis showed that 10 kinds of motifs were identified in these genes,14 BrCNGC proteins contained all of 10 motifs,and the genes that came from the same group shared similar motif composition.The results of quantitative RT-PCR showed that most BrCNGC genes up-regulated and few BrCNGC genes down-regulated by the interaction between plant growth regulator and TuMV.[Conclusions]The structure of BrCNGC in Chinese cabbage is highly conserved and they play a role in the protection against TuMV infection.This finding laid the foundation for the further study on the function of BrCNGC genes.

关 键 词：大白菜 环核苷酸门控离子通道基因(CNGC) 全基因组分析 芜菁花叶病毒(TuMV) 荧光定量PCR 

分 类 号：S634.1[农业科学—蔬菜学]