新疆地区四种草莓病毒病原的检测  

Detection and Investigation of Four Strawberry Viruses in Xinjiang Region

作　　者：杨波[1] 赵宝龙[1] 郝小军[2] 都业娟[2] 何旺 YANG Bo;ZHAO Bao-long;HAO Xiao-jun;DU Ye-juan;HE Wang(College of Agronomy,Shihezi University/Key Laboratory of Cultivation Physiology and Germplasm Resources Utilization of Featured Fruits and Vegetables of Xinjiang Production and Construction Corps, Shihezi Xinjiang 832003,China;Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization at Universities of Xinjiang Uygur Autonomous Region,College of Agronomy, Shihezi University,Shihezi Xinjiang 832003,China)

机构地区：[1]特色果蔬栽培生理与种质资源利用兵团重点实验室/石河子大学农学院,新疆石河子832000 [2]新疆绿洲农业病虫害治理与植保资源利用自治区普通高校重点实验室/石河子大学农学院,新疆石河子832000

出　　处：《新疆农业科学》2018年第9期1689-1697,共9页Xinjiang Agricultural Sciences

基　　金：兵团技术转移专项(2014BD032);兵团科技特派员创新创业计划项目(2015CA011)~~

摘　　要：【目的】研究新疆草莓病毒病病源种类及田间发生情况,建立主要病毒病原多重RT-PCR检测体系,为无病毒草莓生产提供理论和技术支持。【方法】利用已报道的4种草莓主要病毒病原4对特异性引物,分别对新疆主要草莓种植区采集表现疑似病毒病症状的草莓样品共450份,采用单一反转录PCR(RT-PCR)及多重PCR对采集的样品进行检测,分析新疆草莓病毒病的发生情况。【结果】感染新疆草莓有3种主要病毒,南北疆15个不同栽培区均能检测出这3种病毒,每个采集地的病毒检出率均在26%以上。所有样品中病毒总检出率为64. 22%;其中,草莓镶脉病毒(strawberry vein banding virus,SVBV)检出率最高,为51. 78%;草莓斑驳病毒(strawberry mottle virus,SMoV)检出率为23. 78%,草莓轻型黄边病毒(strawberry mild yellow edge virus,SMYEV)检出率为9. 33%,草莓皱缩病毒(Strawberry crinkle virus,SCV)没有检出。病毒复合侵染率达18. 45%,两种病毒病复合侵染检出率为16. 67%,三种病毒病复合侵染检出率为1. 78%;所有样品中对3种病毒的多重RT-PCR与单一RT-PCR检测结果一致。【结论】新疆草莓受SVBV、SMoV和SMYEV三种病毒危害,单一病毒病感染率以及两种以上病毒复合侵染侵染率都较高,在无病毒种苗的培育和生产中加强病毒检测。【Objective】To investigate the origin and occurrence of strawberry virus disease in Xinjiang,and to establish a multiplex RT-PCR detection system for main virus pathogens in the hope of providing theoretical and technical support for the plant production of virus-free strawberry.【Method】According to the 4 pairs of reported specific primers of the 4 species viral disease,450 strawberry samples with suspected viral diseases symptoms were collected from the different main planting areas in Xinjiang,all the samples were tested by single reverse transcription polymerase chain reaction(RT-PCR)and multiplex RT-PCR to analyze the occurrence characteristics of strawberry virus diseases.【Result】The results showed that there were 3 main viruses of SVBV(Strawberry vein banding virus),SMoV(Strawberry mottle virus)and SMYEV(Strawberry mild yellow edge virus),which could be detected from the samples from 15 different cultivation areas in northern and southern Xinjiang and the virus detection rate was above 26%in each collection area.The total detection rate of the virus in all samples was 64.22%,among which,the detection rate of SVBV was the highest(51.78),the detection rate of SmoV was 23.78 and strawberry of SMYEV was 9.33%.Strawberry crinkle virus(SCV)was not detected.The complex infection phenomenon showed that co-infections were up to 18.45%,the detection rate of combined infection of two viruses was 16.67%and the detection rate of combined infection of three kinds of viruses was up to 1.78%.The results of multiplex RT-PCR for three viruses in all samples are consistent with the results of single RT-PCR detection.【Conclusion】Currently the primary viral disease in strawberry growing areas in Xinjiang is caused by SVBV,SMoV and SMYEV and the infection rate of one single virus and two or more viruses are relatively hight.Therefore,so it is necessary to strengthen virus detection in the cultivation and production of virus-free seedlings.

关 键 词：草莓病毒病 RT-PCR 检测 

分 类 号：S668.9[农业科学—果树学] S436.68[农业科学—园艺学]