盐生植物盐角草同化枝不定芽的诱导与增殖  

Adventitious Bud Induction and Multiplication of Assimilation Branch from the Halophyte Salicornia europaea

作　　者：冯肖莉 李学文 王艳[1] FENG Xiao-li;LI Xue-wen;WANG Yan(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2018年第9期1747-1754,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点实验室开放课题"两个HAK/KUP/KT成员的钾转运功能及耐盐性分析"(2017D04026);国家青年自然科学基金"荒漠盐生植物盐穗木转录调控因子HcSCL13的耐盐功能及作用机制研究"(31400729)~~

摘　　要：【目的】建立并确定盐生植物盐角草(Salicornia europaea L)同化枝不定芽诱导与增殖的试验体系和培养基。【方法】以MS为基本培养基,通过附加不同浓度激素(TDZ、NAA、6-BA)及盐(NaCl)进行盐角草不定芽的诱导,筛选诱导盐角草不定芽的最适激素配比及盐浓度;后期通过对细胞分裂素TDZ浓度(0、1. 6、1. 8、2. 0和2. 5 mg/L)的摸索,确定盐穗木不定芽增殖的最适浓度。【结果】盐角草同化枝在含有200 m M NaCl的MS3(2. 0 mg/L TDZ、0. 1 mg/L NAA)培养基中不定芽诱导率达32. 4%,显著高于(P <0. 05)其余组,为最适诱芽培养基。再生的不定芽在含有2. 0 mg/LTDZ的培养基中经过30～40 d的培养,不定芽增殖率可达619. 5%。盐角草不定芽诱导及增殖最适培养基均为:MS+2. 0 mg/L TDZ+0. 1 mg/L NAA+200 m M NaCl。【结论】一定浓度的TDZ、NAA及NaCl组合,可通过直接器官发生途径有效诱导盐角草同化枝不定芽的产生和增殖。【Objective】To establish and confirm the culture system and medium of assimilation branch for the adventitious shoots induction and proliferation from the halophyte Salicornia europaea.【Method】MS medium was used as the basic medium to induce the adventitious buds of S.europaea by adding different concentration hormones(TDZ,NAA,6-BA)and salt(NaCl)to screen the optimum hormone ratio and salt concentration.In the later stage,we set different concentration of cytokinin TDZ(0,1.6,1.8,2.0 2.5 mg/L)to determine the optimal proliferation culture medium of adventitious buds in S.europaea.【Result】The results showed that the induction rate of adventitious buds in the medium of MS 3(2.0 mg/L TDZ,0.1 mg/L NAA)containing 200 mM NaCl was 32.4%,which was significantly higher than those of other groups(P<0.05)and acted as the most appropriate induction of adventitious bud culture medium.The regenerated adventitious buds were cultured in a medium containing 2.0 mg/LTDZ for 30-40 days,and the multiplication rate of adventitious buds reached 619.5%.Both optimum media for adventitious bud induction and proliferation of S.europaea were MS+2.0 mg/L TDZ+0.1 mg/L NAA+200 mM NaCl.【Conclusion】It can be seen that different concentrations of TDZ,NAA and NaCl can effectively induce adventitious buds and promote the proliferation of adventitious buds through the direct organogenesis pathway.

关 键 词：盐角草 同化枝 不定芽诱导 增殖 

分 类 号：Q943.2[生物学—植物学] S188[农业科学—农业基础科学]