胆木提取液与抗生素配伍对金黄色葡萄球菌的抑制效果  

Inhibitory Effect of Nauclea officinalis Pierre ex Pitard.Extract and Different Antibiotic Combinations on Staphylococcus aureus

作　　者：蒋平 李小慧 高群 朱寒 鲍丽莉 左瑞华[1,2] JIANG Ping;LI Xiaohui;GAO Qun;ZHU Han;BAO Lili;ZUO Ruihua(College of Biology and Pharmaceutical Engineering,West Anhui University,Lu’an,Anhui 237012;The Monitoring and Early Warning Engineering Center for Healthy Breeding and Animal Epidemic Disease,West Anhui University,Lu’an,Anhui 237012,China)

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]皖西学院健康养殖与动物疫病监测预警工程技术中心,安徽六安237012

出　　处：《贵州农业科学》2018年第11期79-82,共4页Guizhou Agricultural Sciences

基　　金：安徽省本科质量工程项目(2016sjjd079);皖西学院教学研究项目(wxxy2017078);皖西学院林下种草养鹅生态养殖模式与示范项目(WXZR201743);皖西学院研究性学习项目(wxxyx2018083;wxxyx2018103)

摘　　要：探明常用抗生素与胆木提取液配伍对金黄色葡萄球菌的抑菌效果,为兽医临床应用提供一定的借鉴与理论参考,以胆木提取液、青霉素、左氧氟沙星、磷霉素钠、头孢拉定为试验药物,以金黄色葡萄球菌为试验菌种,采用倍比稀释法检测5种药物的最小抑菌浓度,棋盘稀释法检测胆木提取液分别与青霉素、左氧氟沙星、磷霉素钠和头孢拉定4种抗生素联用对金黄色葡萄球菌的联合抑菌效果。结果表明:胆木提取液、青霉素、左氧氟沙星、磷霉素钠和头孢拉定的最小抑菌浓度分别为40.00 mg/mL、0.25μg/mL、0.50μg/mL、0.50μg/mL和1.00μg/mL。胆木提取液与青霉素、头孢拉定、左氧氟沙星和磷霉素联用后分别表现为协同作用、相加作用、无关作用和拮抗作用。胆木提取液与青霉素联用对金黄色葡萄球菌具有高效的抑制作用。The minimal inhibitory concentration(MIC)of Nauclea officinalis Pierre ex Pitard.extract,Penicillin,Levofloxacin,Fosfomycin Sodium and Cefradine to Staphylococcus aureus was detected by using a doubling dilution method and the combined antibacterial effect of N.officinalis Pierre ex Pitard.extract+Penicillin combination,N.officinalis Pierre ex Pitard.extract+Levofloxacin combination,N.officinalis Pierre ex Pitard.extract+Fosfomycin Sodium combination and N.officinalis Pierre ex Pitard.extract+Cefradine combination to S.aureus was respectively detected by a checkerboard assay to prove the antibacterial effect of N.officinalis Pierre ex Pitard.extract and routine antibiotics combinations on S.aureus and provide the theoretical references for veterinary clinical application.Result:The minimal inhibitory concentration(MIC)of N.officinalis Pierre ex Pitard.extract,Penicillin,Levofloxacin,Fosfomycin Sodium and Cefradine to S.aureus is 40.00 mg/mL,0.25μg/mL,0.50μg/mL,0.50μg/mL and 1.00μg/mL respectively.The combined antibacterial effect of N.officinalis Pierre ex Pitard.extract+Penicillin combination,N.officinalis Pierre ex Pitard.extract+Cefradine combination,N.officinalis Pierre ex Pitard.extract+Levofloxacin combination and N.officinalis Pierre ex Pitard.extract+Fosfomycin Sodium combination to S.aureus is synergistic action,additive action,indifference action and antagonistic action separately.The N.officinalis Pierre ex Pitard.extract+Penicillin combination has an efficient inhibitory effect on S.aureus.

关 键 词：胆木提取液 抗生素 配伍 金黄色葡萄球菌 抑制效果 

分 类 号：S853.7[农业科学—临床兽医学] R2-031[农业科学—兽医学]