具有近红外光热转换性能的双层氧化硅包覆金纳米花复合材料的制备及体内代谢研究  

Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo

作　　者：宋文植[1] 李慧[1] 张艳[1] 何丹[2] 李英姿[1] 黄臻臻 刘新[1] 尹万忠[2] SONG Wenzhi;LI Hui;ZHANG Yan;HE Dan;LI Yingzi;HUANG Zhenzhen;LIU Xin;YIN Wanzhong(The First Clinical Hospital of Jilin University,Changchun 130021,China;China-Japan Union Hospital of Jilin University,Changchun 130031,China;College of Chemistry,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学中日联谊医院,长春130031 [2]吉林大学第一临床医院,长春130021 [3]吉林大学化学学院,长春130012

出　　处：《高等学校化学学报》2018年第12期2644-2650,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:81372900);吉林省科技发展计划项目(批准号:20110708,20180414014GH);2014年吉林大学交叉创新择优资助项目资助~~

摘　　要：采用种子生长法和改良Stber法制备了双层氧化硅包覆的金纳米花复合材料,并测试了其近红外光热转换性能,研究了其在裸鼠体内的生物毒性,并以种植了人口腔鳞癌CAL27细胞的荷瘤裸鼠为体内模型,应用光声成像方法研究了其体内分布、代谢和在肿瘤组织的被动靶向富集行为.结果表明,所制备的金纳米花复合材料在近红外光区具有良好的光热转换性能,无明显体内生物毒性,是一种良好的光声成像剂,其在荷瘤裸鼠体内主要经肝脏代谢至肠道排出体外,可通过EPR效应实现在肿瘤组织的靶向富集.本研究证明所制备的双层氧化硅包覆金纳米花复合材料可用于生物体内治疗,可作为包括口腔癌在内的恶性肿瘤近红外光热诊疗的理想介导材料.Gold nanoflower-double layer silica core-shell nanoparticles were prepared by a seed-mediated growth strategy and a modified St ber method.The photothermal properties and the biotoxicity in vivo of the as-prepared core-shell nanoparticles were studied.The nude mice bearing oral cancer cell CAL27 were taken as the animal model to investigate the distribution,metabolism and passive targeting enrichment of the core-shell nanoparticles in vivo by the photoacoustic imaging technique.The results showed that the as-prepared core-shell nanoparticles exhibited excellent photothermal conversion properties in near infrared(NIR)region.The core-shell nanoparticles could be well metabolized in the liver,and could be excreted outside of the body through the intestinal tract.More importantly,the core-shell nanoparticles exhibited passive targeting enrichment in the tumor tissue through EPR effect.Our study show that the gold nanoflower-double layer silica core-shell nanoparticles would be an ideal photoacoustic imaging agent and a NIR photothermal therapy agent for diagnose and therapy of malignant tumors such as oral cancer.

关 键 词：金纳米花-二氧化硅复合粒子 核壳结构纳米粒子 光声成像 近红外光热转换 口腔癌 

分 类 号：O614[理学—无机化学] O644[理学—化学]