水溶性超分子荧光探针对多菌灵的识别及细胞成像  

Water-soluble Supramolecular Fluorescent Probe for Sensing Carbendazim and Its Application in Living Cell Imaging

作　　者：杨梅 刘青[2] 唐青[3] 王成会 杨梅香 孙涛 黄英[5,2] 陶朱 YANG Mei;LIU Qing;TANG Qing;WANG Chenghui;YANG Meixiang;SUN Tao;HUANG Ying;TAO Zhu(The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China;Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province;Key Laboratory of Guizhou High Performance Computational Chemistry;College of Tobacco Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学西南药用生物资源教育部工程研究中心,贵阳550025 [2]贵州大学贵州省大环化学及超分子化学重点实验室,贵阳550025 [3]贵州大学烟草学院,贵阳550025 [4]贵州大学贵州省高性能计算化学重点实验室,贵阳550025 [5]贵州大学贵州大学西南药用生物资源教育部工程研究中心,贵阳550025

出　　处：《高等学校化学学报》2018年第12期2665-2672,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21562015);贵州省优秀青年科技人才培养计划(批准号:黔教合平台人才[2017]5636);贵州省普通高等学校科技拔尖人才支持计划项目(批准号:黔教合KY字[2016]059);贵州省科技厅自然科学基金重点项目(批准号:黔教合JZ字[2014]2005);贵州省科技厅联合基金项目(批准号:黔教合LH字[2014]7658);教育部春晖计划合作科研项目(批准号:Z2015002)资助

摘　　要：基于八元瓜环(Q[8])可使吖啶橙(AD)的荧光降低的性质制备了荧光探针2AO@Q[8],当在该探针中加入多菌灵后荧光强度又逐渐增强,利用此超分子配合物的荧光效应,构建了一种能够检测多菌灵的超分子荧光探针.研究结果表明,该探针在水溶液中对多菌灵具有良好的选择性,在一定浓度范围内其线性关系良好,检出限为8. 14×10^(-8)mol/L.细胞成像结果显示,该探针在前列腺癌细胞中对多菌灵具有良好的响应,可用于生物细胞内多菌灵的识别检测.A host-guest complex between cucurbit[8]uril(Q[8])and acridine orange(AO)was used as a fluorescent probe for detecting the carbendazim(CBZ).2AO@Q[8]fluorescent probe was designed and used to detect carbendazim with high sensitivity and selectivity with a detection limit of ca.10^-8 mol/L.A good linear relationship of emission intensity at ca.529 nm for CBZ at concentrations of 0.4-5.0μmol/L was observed.The proposed sensing mechanism was investigated via 1H NMR spectroscopy combined with density functional theory calculations at the B3LYP/6-31G(d)level.The cell imaging study showed that the 2AO@Q[8]complex could be used to detect carbendazim in prostate cancer(PC3)cells,which may help to sense carbendazim in biological system.

关 键 词：八元瓜环 吖啶橙 荧光探针 多菌灵 细胞成像 

分 类 号：O657[理学—分析化学]