益母草碱对多柔比星致小鼠肾损伤的保护作用  

Protective effect of leonurine on adriamycin-induced kidney injury in mice

作　　者：孙涛 袁斌 SUN Tao;YUAN Bin(Department of Paediatrics,Affiliated Hospital of Nanjing University of TCM,Nanjing 210029,Jiangsu,China)

机构地区：[1]南京中医药大学附属医院儿科,南京医学硕士研究生210029

出　　处：《医学研究生学报》2018年第12期1267-1271,共5页Journal of Medical Postgraduates

摘　　要：目的益母草碱具有降脂、降低血黏度、改善微循环等生物学功能。文中探讨益母草碱对多柔比星肾病小鼠肾的保护作用机制。方法将30只雄性Balb/C小鼠采用随机数字表法分为正常组(n=10)、模型组(n=10)和治疗组(n=10)。模型组和治疗组一次性尾静脉注射多柔比星5.0 mg/kg,正常组注射等量等渗盐水。造模后第1天开始,治疗组予益母草碱50 mg/(kg·d)灌胃,正常组和模型组予等量蒸馏水灌胃,共治疗6周后检测各组小鼠24 h尿蛋白定量、三酰甘油、血尿素、血肌酐、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)水平,ELISA法定量分析各组小鼠血清中转化生长因子(TGF)-β、IL-18、干扰素(IFN)-γ水平,并使用Western blot法检测SMAD2/3的磷酸化水平。结果与正常组比较,模型组尿蛋白、血肌酐、血尿素氮、三酰甘油及TGF-β、IL-18蛋白表达水平明显升高,差异有统计学意义(P<0.05),SMAD3的磷酸化水平及其靶蛋白α-SMA显著升高,差异均有统计学意义(P<0.05)。与模型组比较,益母草碱组尿蛋白、血肌酐、血尿素氮、三酰甘油水平以及MDA、TGF-β、IL-18、IFN-γ蛋白表达显著降低,SOD与GSH显著升高,差异均有统计学意义(P<0.05)。结论益母草碱具有改善多柔比星引起的肾损害,其作用机制可能与其抑制多柔比星造成的肾氧化应激以及炎症有关。Objective Leonurine(LEO)has shown biological effects such as reducing fat,lowering blood viscosity,and improving microcirculation.The article aimed to investigate the protective effect of LEO on the kidney of mice with adriamycin nephropathy(AN). Methods A total of 30 male mice were randomly assigned into normal control group(n=10),model group(n=10),and LEO group(n=10).Mice in model group and LEO group were injected with 5.0mg/kg adriamycin via caudal vein to induce nephropathy.The same volume of isotonic saline was injected in control group.At 1d after modeling,mice in LEO group were given 50mg/(kg·d)LEO by gavage,and the same volume of distilled water was given by gavage in control group and model group for 6 consecutive weeks.Measurement was made on 24 hour urinary albumin,plasma triglyceride,creatinine,blood urea nitrogen(BUN),SOD,GSH,MDA.Elisa was applied in testing serum TGF-β,IL-18 and IFN-γand western blot was used to examine the phosphorylation of SMAD2/3. Results Compared with control group,the significant exaltation in the expression of TGF-β、IL-18 and IFN-γ,urinary albumin,plasma triglyceride,creatinine,blood urea nitrogen(BUN),MDA and the phosphorylation of SMAD3 and the target protein of SMAD3(α-SMA,fibronectin)were elevated in model group(P<0.05);while the indexes were obviously decreased in LEO group than those in model group(P<0.05). Conclusion LEO can improve the renal pathological damage induced by adriamycin,whose mechanism may be related to the inhibition of oxidative stress and inflammation.

关 键 词：益母草碱 多柔比星肾病 超氧化物歧化酶 谷胱甘肽 转化生长因子-Β 

分 类 号：R692[医药卫生—泌尿科学]