小麦条锈病菌夏孢子显微图像远程采集系统设计与试验  

Design and Experiment of Micro-image Remote Acquisition System of Uredinispores of Puccinia striiformis f. sp. tritici

作　　者：雷雨[1,2] 姚志凤 何东健[1,2] LEI Yu;YAO Zhifeng;HE Dongjian(College of Mechanical and Electronic Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China;Key Laboratory of Agricultural Internet of Things,Ministry of Agriculture and Rural Affairs,Yangling,Shaanxi 712100,China;Shaanxi Key Laboratory of Agricultural Information Perception and Intelligent Service,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学机械与电子工程学院,陕西杨凌712100 [2]农业农村部农业物联网重点实验室,陕西杨凌712100 [3]陕西省农业信息感知与智能服务重点实验室,陕西杨凌712100

出　　处：《农业机械学报》2018年第11期39-47,共9页Transactions of the Chinese Society for Agricultural Machinery

基　　金：陕西省重点产业链项目(2015KTZDNY01-06);陕西省科技攻关项目(2017NY-104)

摘　　要：利用孢子捕捉器捕捉小麦田间空气中的条锈病菌夏孢子已成为当前小麦条锈病预测预报的重要手段。针对现有的孢子捕捉设备效率低、费时费力、需人工定时换取载玻片或捕捉带等问题,基于ARK-1123C型嵌入式工控机和显微镜CCD数字摄像头,提出了一种高放大倍数、高分辨率的小麦条锈病菌夏孢子显微图像远程采集系统的设计方案。设计了系统的硬、软件结构,实现了自动取载玻片、涂脂、空中孢子捕捉、孢子显微图像采集、载玻片回收等一系列功能,且可根据用户需求远程设置孢子捕捉和显微图像采集参数,采集的图像通过无线网络传输到远程服务器中。为了验证系统的性能,在小麦田间进行了40 d的系统综合试验测试。测试结果表明,系统可长时间稳定工作,能够远程实时采集放大400倍的4 096像素×3 288像素的夏孢子显微图像。该系统能够实时采集和远程传输小麦条锈病菌夏孢子显微图像,可满足野外小麦田间空气中夏孢子监测的需求,为农田空气中小麦条锈病菌夏孢子的自动计数及条锈病的预测预报提供重要技术支持。Collecting airborne urediniospores of Puccinia striiformis f.sp.tritici(Pst)in wheat fields using spore trap devices has currently become an important approach for devising strategies early and effectively controlling wheat stripe rust.However,the existing spore trap devices have some shortcomings,such as low efficiency,time-consuming and requiring manual replacement of slides or plastic tapes.To this end,a high-magnification and high-resolution micro-image remote acquisition system of uredinispores of Puccinia striiformis f.sp.tritici was designed and manufactured based on ARK 1123C embedded industrial computer and microscope CCD digital camera.The hardware and software architectures of the micro-image remote acquisition system were designed,which realized a series of functions,including automatic supply of slide,coating a thin film of petroleum jelly,aerial urediniospore capture,urediniospore micro-image acquisition,and slide recycling.Besides,the parameters of urediniospore capture and micro-image acquisition could be set remotely according to user’s requirement,and the collected images could be transmitted to the remote server by 4G wireless network.In order to verify the performance of the system,the system was deployed and tested in a wheat field for 40 d.Experimental results showed that the system worked stably for a long time,which could capture the micro-images of uredinispores,with 400 times magnification and 4 096 pixels×3 288 pixels resolution.The experiments validated that the proposed remote acquisition system can automatically collect and remotely transmit the microscopic image of uredinispores in real time,which provided important technical support for the automatic counting of the airborne uredinispores and the prediction of the wheat stripe rust in the field.It can also provide a reference for monitoring other airborne fungal spores.

关 键 词：小麦条锈病 夏孢子 显微图像 远程采集系统 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治] TP23[农业科学—植物保护]