MicroRNA-200c对Ox-LDL介导的人主动脉内皮细胞损伤的影响  

Effect of miR-200c on Ox-LDL-induced injury of human aortic endothelial cells

作　　者：朱伟军[1] 雷军荣 白晓君[3] 王蕊[1] 叶玉兰[1] 鲍建军 Wei-jun Zhu;Jun-rong Lei;Xiao-jun Bai;Rui Wang;Yu-lan Ye;Jian-jun Bao(Department of Cardiology,Xi’an Gaoxin Hospital,Xi’an,Shaanxi 710075,China;Department of Cardiothoracic Surgery,Xi’an Gaoxin Hospital,Xi’an,Shaanxi 710075,China;Department of Cardiology,First Affiliated Hospital,Medical College of Xi'an Jiaotong University,Xi'an,Shaanxi 710061,China)

机构地区：[1]陕西省西安高新医院心内科,陕西西安710075 [2]陕西省西安高新医院心胸外科,陕西西安710075 [3]西安交通大学医学院第一附属医院心血管内科,陕西西安710061

出　　处：《中国现代医学杂志》2018年第34期11-16,共6页China Journal of Modern Medicine

摘　　要：目的探讨micro RNA-200c(mi R-200c)在氧化低密度脂蛋白(Ox-LDL)损伤人主动脉内皮细胞(HAEC)中的表达及其对细胞活力、凋亡的影响及相关机制。方法采用q RT-PCR检测Ox-LDL损伤HAEC mi R-200c的表达水平;转染mi R-200c inhibitor抑制miR-200c的表达后,分别运用MTT和流式细胞术检测细胞活力和细胞凋亡情况;Western blot检测Slit2蛋白表达水平;荧光素酶报告系统检测mi R-200c对Slit2的靶向调节。结果 Ox-LDL呈浓度和时间模式刺激HAEC中mi R-200c表达上调。抑制mi R-200c表达可上调细胞活力,抑制Ox-LDL诱发的细胞凋亡。抑制mi R-200c可上调Slit2蛋白表达水平。双荧光素酶报告实验转染In-mi R-200c的细胞内Slit2相对荧光强度高于对照组。沉默Slit2可部分逆转In-mi R-200c对细胞活力的促进作用和对细胞凋亡的抑制作用。结论 mi R-200c在Ox-LDL诱导的HAEC表达上调,抑制mi R-200c的表达可通过靶向作用于Slit2抑制Ox-LDL诱导的细胞凋亡。Objective To investigate the expression and effect of miR-200c on cell survival and apoptosis in human aortic endothelial cells(HAECs)injured by oxidized low density lipoprotein(Ox-LDL).Methods The expression of miR-200c in Ox-LDL injured HAECs was detected by qRT-PCR.After transfection of miR-200c inhibitor,the cell viability and cell apoptosis were analyzed by MTT and flow cytometry respectively.Luciferase assay was performed to detect whether Slit2 was the direct target of miR-200c.Results miR-200c expression was up-regulated by Ox-LDL treatment in the HAECs in a time-and dose-dependent manner.miR-200c inhibition enhanced cell survival and inhibited cell apoptosis.Knockdown of miR-200c up-regulated Slit2 protein expression level.The transfection of miR-200C inhibitor significantly increased the relative luciferase intensity of Slit2.Furthermore,silencing of Slit2 partly reversed the effect of miR-200c inhibitor on cell survival and cell apoptosis.Conclusions miR-200c is up-regulated in Ox-LDL-treated HAECs,and miR-200c inhibition impedes Ox-LDLinduced apoptosis by targeting Slit2.

关 键 词：动脉粥样硬化 微RNAS 细胞增殖 细胞凋亡 脂蛋白类 低密度脂蛋白 SLIT2 

分 类 号：R543[医药卫生—心血管疾病]