白藜芦醇对急性心肌梗死大鼠心脏神经重塑的影响及其机制  

Mechanism of Resveratrol in Neural Remodeling in the Rat Heart Following Acute Myocardial Infarction

作　　者：姜晓晓[1] 周祖华 常栋[3] 齐中华[4] JIANG Xiaoxiao;ZHOU Zuhua;CHANG Dong;QI Zhonghua(Department of Cardiac Surgery,The First Affiliated Hospital,Dalian Medical University,Dalian 116011,China;Department of Outpatient,Liaoning Province Military Region Dalian Sixteenth Retired Cadres,Dalian 116041,China;Department of Cardiology,The First Affiliated Hospital,Dalian Medical University,Dalian 116011,China;Department of Neurology,The First Affiliated Hospital,Dalian Medical University,Dalian 116011,China)

机构地区：[1]大连医科大学附属第一医院心脏外科,辽宁大连116011 [2]辽宁省军区大连第十六离职干部休养所门诊部,辽宁大连116041 [3]大连医科大学附属第一医院心内科,辽宁大连116011 [4]大连医科大学附属第一医院神经内科,辽宁大连116011

出　　处：《中国医科大学学报》2018年第12期1077-1080,共4页Journal of China Medical University

基　　金：国家自然科学基金(81200136)

摘　　要：目的探讨白藜芦醇对心肌梗死大鼠心脏神经重塑的影响及其作用机制。方法 Sprague-Dawley大鼠随机分为3组:假手术组(n=10,缝线穿过冠状动脉前降支下方但不结扎)、手术组(n=10,结扎冠状动脉前降支)、手术给药组(n=10,结扎冠状动脉前降支,并给予白藜芦醇)。手术后4周,Western blotting和实时PCR检测心肌组织中神经生长因子和白细胞介素1β蛋白、mRNA的表达情况。结果与假手术组比较,手术组、手术给药组梗死周边区心肌组织中神经生长因子和白细胞介素1β蛋白和mRNA表达水平明显升高(均P <0.05);与手术组比较,手术给药组梗死周边区心肌组织中神经生长因子和白细胞介素1β蛋白和m RNA表达水平明显降低(均P <0.01)。结论白藜芦醇可减少心肌梗死大鼠心肌组织中的神经生长因子、白细胞介素1β蛋白和mRNA的表达,进而抑制心脏神经重塑,预防室性心律失常和心源性猝死。Objective To explore the mechanism of resveratrol in neural remodeling in the infarcted rat myocardium.Methods Rats were randomly divided into 3 groups:a sham group(n=10),in which sutures were passed but not tied;a surgery group(n=10),in which the anterior descending artery was ligated;and a resveratrol group(n=10),in which the anterior descending artery was ligated combined with resveratrol injection.At four weeks after surgery,the level of nerve growth factor(NGF)and interleukin-1β(IL-1β)at the border zone was measured with Western blotting;the expression of NGF and IL-1β mRNA was examined with real-time polymerase chain reaction.Results Compared with the sham group,the level of NGF and IL-1β in the surgery group was significantly increased(P<0.01).Compared with the sham group,the level of NGF and IL-1β in the resveratrol group was significantly increased(P<0.05).Resveratrol significantly reduced the expression of NGF and IL-1β compared with that in the surgery group(P<0.01).Conclusion After acute myocardial infarction,resveratrol reduced the expression of NGF protein and mRNA in rats.Resveratrol also decreased the expression of IL-1βprotein and mRNA.Our findings suggest that resveratrol can prevent ventricular arrhythmia and sudden cardiac death by inhibiting cardiac neural remodeling.

关 键 词：白藜芦醇 心肌梗死 神经重塑 神经生长因子 白细胞介素1Β 大鼠 

分 类 号：R654.2[医药卫生—外科学]