杜鹃远缘杂交后代的无菌培养研究  

Study on the Aseptic Culture of Hybridized Offspring of Rhododendron

作　　者：资宏 杨婧瑜 方林川 周媛 ZI Hong;YANG Jing-yu;FANG Lin-chuan;ZHOU Yuan(Forestry Research Institute,Wuhan Academy of Agricultural Sciences/Hubei Provincial Garden Plant Engineering Technology Research Center,Wuhan 430070,China;College of Horticulture&Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]武汉市农业科学院林业果树研究所/湖北省园林植物工程技术研究中心,武汉430070 [2]华中农业大学园艺林学学院,武汉430070

出　　处：《湖北农业科学》2018年第22期90-95,共6页Hubei Agricultural Sciences

基　　金：国家自然科学基金青年项目(31601792);国家花卉联盟项目(2016hhlm005)

摘　　要：选用杜鹃(Rhododendron)品种♂胭脂蜜(Rhododendron obtusum ‘Yanzhimi’)×♀春五宝(Rhododendron hybridum ‘Chunwubao’)杂交种子作为试验材料,研究了不同杀菌方式、不同培养基初代培养、不同生长调节剂、低温处理及光照条件对种子萌发与生长以及添加不同浓度生长调节剂的1/2 WPM培养基对杂交无菌苗生根率的影响。结果表明,杜鹃杂交种子的最佳杀菌方式为1.5%NaClO溶液处理10min+75%乙醇处理30 s;在经过为期20 d的4℃低温处理,并添加浓度为100 mg/L GA3的1/2 WPM培养基中培养杜鹃杂交种子,其发芽率最高,达到56.7%;光照对杜鹃杂交种子的萌发起促进作用,光照处理下的种子萌发率高于黑暗处理,且种子萌发的时间提前3～7 d;添加了0.5 mg/L NAA、0.1 mg/L IBA的1/2 WPM培养基使杜鹃杂交无菌苗的生根率达到了最佳。Taking Rhododendron cultivar"Rhododendron obtusum‘Yanzhimi’×Rhododendron hybridum‘Chunwubao’"as experimental materials,the effects of different methods of disinfection,initial culture of different kinds of media,different plant growth regulators,low temperature treatment and light conditions on seed germination and the growth were studied,and the effects of 8 kinds of 1/2 WPM medium supplemented with different concentrations of plant growth regulators on the rooting rate of hybrid sterile seedlings were also studied.The results showed that the optimum disinfection method for Rhododendron hybrid seeds was 1.5%NaClO solution for 10 min and 75%ethanol for 30 s.On the condition of 20 days low-temperature treatments at 4℃,and culturing Rhododendron hybrid seed with 100 mg/L GA3 in 1/2 WPM medium,the highest germination rate was got,and the value was 56.7%.Light irradiation promoted the germination of Rhododendron hybrid seed,the germination rate of seed under light treatment was higher than that of dark treatment,and the seed germination time was also 3~7 days earlier.On the condition of 1/2 WPM medium with 0.5 mg/L NAA and 0.1 mg/L IBA,Rhododendron hybrid sterile seedling had the highest rooting rate.

关 键 词：杜鹃(Rhododendron) 低温 无菌播种 发芽率 生根率 杂交不亲和 

分 类 号：S681.9[农业科学—观赏园艺]