独一味对糖尿病痛大鼠脊髓背角内胶质细胞激活的影响  

Effects of Lamiophlomis rotata on glial cell activation in spinal dorsal horn of rats with diabetic pain

作　　者：谢菁 聂琼芳[1] 吴小兰[1] XIE Jing;NIE Qiong-fang;WUXiao-lan(Wuhan University of Technology,Wuhan 430010,China)

机构地区：[1]武汉理工大学,湖北武汉430010

出　　处：《中成药》2018年第12期2624-2629,共6页Chinese Traditional Patent Medicine

摘　　要：目的考察独一味对糖尿病痛大鼠脊髓背角内胶质细胞激活的影响。方法 70只大鼠随机分成正常组、枸橼酸-独一味组、链脲佐菌素-安慰剂组、链脲佐菌素-独一味组(30、224、300、3 000 mg/kg),每组10只。链脲佐菌素枸橼酸缓冲液建立糖尿病痛大鼠模型,机械痛刺激法测定大鼠后足爪痛阈,免疫荧光染色、Western blot法检测大鼠脊髓背角内Iba-1、GFAP、p-STAT3表达水平。结果与链脲佐菌素-安慰剂组比较,链脲佐菌素-独一味组(30、300、3 000 mg/kg)痛阈有所增加,并呈明显的剂量依赖性(P <0. 05),在300、3 000 mg/kg剂量下更显著(P <0. 01);链脲佐菌素-独一味组(224 mg/kg) Iba-1、GFAP阳性细胞数显著减少(P <0. 01),蛋白表达显著降低(P <0. 01),p-STAT3表达水平也显著降低(P <0. 01)。结论独一味可有效缓解糖尿病痛大鼠疼痛反应,其机制可能与抑制大鼠脊髓背角内胶质细胞激活、降低p-STAT3表达、改善炎症有关。AIM To investigate the effects of Lamiophlomis rotata(Benth.) Kudo on glial cell activation in spinal dorsal horn of rats with diabetic pain. METHODS Seventy rats were randomly divided into normal group,citric acid-L. rotata group, streptozocin-placebo group and streptozocin-L. rotata groups(30, 224, 300,3 000 mg/kg),ten rats in each group. The rat model for diabetic pain was established by streptozotocin-citric acid buffer solution. Mechanical pain stimulus method was applied to determining the pain threshold of rat hind paw.Immunofluorescence staining and Western blot were used to detect the expression levels of GFAP,Iba-1 and pSTAT3 in rat spinal dorsal horn. RESULTS Compared with the streptozocin-placebo group,the streptozocin-L.rotata groups(30,300,3 000 mg/kg) shared dose-dependent increase of pain threshold(P < 0. 05),especially at the doses of 300,3 000 mg/kg(P < 0. 01); marked reduction of Iba-1,GFAP positive cell counts(P < 0. 01)at dose concentration of 224 mg/kg,and significant suppression of protein expressions(P < 0. 01),and p-STAT3 expression level(P < 0. 01). CONCLUSION L. rotata can effectively alleviate the pain reactions of rats with diabetic pain,whose mechanisms may be related to activation inhibition of glial cells,expression suppression of pSTAT3 and thus the inflammation control in the spinal dorsal horn of rats.

关 键 词：独一味 糖尿病痛 胶质细胞 脊髓背角 

分 类 号：R285.5[医药卫生—中药学]