HPLC法同时测定乌金止痛丸中5种成分  

Simultaneous determination of five constituents in Wujin Zhitong Pills by HPLC

作　　者：李燕 邓昭君 焦宝元 LI Yan;DENG Zhao-jun;JIAO Bao-yuan(Shanghai Jinpibao Pharmaceutical Co.,Ltd.,Shanghai 200080,China;Guangzhou Boji Medical Biotechnological Co.,Ltd.,Guangzhou 510000,China;Guangdong Taiantang Pharmaceutical Co.,Ltd.,Shantou 515064,China)

机构地区：[1]上海金皮宝制药有限公司,上海200080 [2]广州博济医药生物技术股份有限公司,广东广州510000 [3]广东太安堂药业股份有限公司,广东汕头515064

出　　处：《中成药》2018年第12期2681-2684,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法同时测定乌金止痛丸(大黄、当归、香附等)中5种成分的含有量。方法该药物50%乙醇提取液的分析采用Amethyst C18-H色谱柱(4. 6 mm×250 mm,5μm);流动相甲醇-0. 05%三氟乙酸,梯度洗脱;体积流量1. 0 mL/min;柱温30℃;检测波长254 nm。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0. 805 4～80. 540 1、0. 815 3～81. 525 3、0. 822 2～82. 217 1、0. 859 5～85. 954 8、0. 382 5～38. 253 6μg/mL范围内线性关系良好(r=0. 999 9),平均加样回收率分别为94. 31%、96. 77%、101. 14%、91. 64%、97. 27%,RSD分别为3. 66%、3. 91%、1. 84%、3. 99%、1. 69%。结论该方法准确,重复性好,可用于乌金止痛丸的质量控制。AIM To establish an HPLC method for the simultaneous content determination of five constituents in Wujin Zhitong Pills(Rhei Radix et Rhizoma,Angelicae sinensis Radix,Cyperi Rhizoma,etc.). METHODS The analysis of 50% ethanol extract of this drug was performed on a 30 ℃ thermostatic Amethyst C18-H column(4. 6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-0. 05% trifluoroacetic acid flowing at1. 0 mL/min in a gradient elution manner,and the detection wavelength was set at 254 nm. RESULTS Aloeemodin,rhein,emodin,chrysophanol and physcion showed good linear relationships within the ranges of 0. 805 4-80. 540 1,0. 815 3-81. 525 3,0. 822 2-82. 217 1,0. 859 5-85. 954 8,0. 382 5-38. 253 6 μg/mL(r =0. 999 9),whose average recoveries were 94. 31%,96. 77%,101. 14%,91. 64%,97. 27% with the RSDs of3. 66%,3. 91%,1. 84%,3. 99%,1. 69%,respectively. CONCLUSION This accurate and reproducible method can be used for the quality control of Wujin Zhitong Pills.

关 键 词：乌金止痛丸 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 HPLC 

分 类 号：R927.2[医药卫生—药学]