环孢素A和他克莫司在体外对外周血单个核细胞内乙型肝炎病毒复制的影响  

In vitro effect of ciclosporin A and tacrolimus on the replication of peripheral blood mononuclear cells mesne hepatitis B virus

作　　者：张友福[1] 罗来邦[1] 杨华[1] 丁利民[1] 杨锦然 罗文峰[1] 龙成美[1] 李新长[1] ZHANG Youfu;LUO Laibang;YANG Hua;DING Limin;YANG Jinran;LUO Wenfeng;LONG Chengmei;LI Xinchang(Department of No.3 General Surgery,the People’s Hospital of Jiangxi Province,Nanchang 331100,China)

机构地区：[1]江西省人民医院普外三科,南昌331100

出　　处：《上海医药》2018年第23期97-99,共3页Shanghai Medical & Pharmaceutical Journal

基　　金：江西省卫生计生委科技计划(项目编号:20165026)

摘　　要：目的:探讨环孢素A(ciclosporin A, CsA)和他克莫司(FK506)在体外对外周血单个核细胞内乙型肝炎病毒复制的影响。方法:建立乙型肝炎病毒脱氧核糖核酸(HBV-DNA)永久转染人外周血单个核细胞株,计算CsA和FK506对HBV-DNA永久转染人外周血单个核细胞株的药物安全浓度,采用狭缝印迹杂交法半定量分析细胞内HBVDNA水平。结果:CsA和FK506的药物作用安全浓度分别是≤36μg/ml和≤200 ng/ml。在安全浓度范围内,CsA的质量浓度与细胞内HBV-DNA相对复制水平呈显著负相关(r=-0.886,P <0.05);FK506的质量浓度与细胞内HBVDNA相对复制水平无明显相关性(r=-0.593,P>0.05)。结论:CsA在体外呈剂量依赖性地抑制外周血单个核细胞内乙型肝炎病毒复制,而FK506不具备上述特性。Objective:To discuss the effect of ciclosporin A(CsA)and tacrolimus(FK506)on the replication of peripheral blood mononuclear cells mesne hepatitis B virus in vitro.Methods:A human peripheral blood mononuclear cell line permanently transfected with hepatitis B virus deoxyribonucleic acid(HBV-DNA)was established to calculate the safe concentrations of CsA and FK506 for HBV-DNA permanently transfected human peripheral blood mononuclear cell line,and semi-quantitative analysis of intracellular HBV-DNA levels was performed by slot blot hybridization.Results:The safe concentrations of CsA and FK506 were<36 mg/ml and<200 ng/ml,respectively.Within the safe concentration range,the mass concentration of CsA was negatively correlated with the relative replication level of HBV-DNA in cells(r=-0.886,P<0.05);the mass concentration of FK506 was not correlated with the relative replication level of HBV-DNA in cells(r=-0.593,P>0.05).Conclusion:CsA can in vitro inhibit hepatitis B virus replication in peripheral blood mononuclear cells in a dose-dependent manner but not FK506.

关 键 词：环孢素A 他克莫司 乙型肝炎病毒 外周血单个核细胞 

分 类 号：R373.21[医药卫生—病原生物学]