蛋白磷酸化修饰在保卫细胞响应非生物胁迫中的作用机制  

Insights of Protein Phosphorylation Modification in Stomatal Guard Cells under Abiotic Stress

作　　者：尹赜鹏 莫江玲 齐明芳[1] 孟思达 李天来[1] YIN Ze-peng;MO Jiang-ling;QI Ming-fang;MENG Si-da;LI Tian-lai(College of Horticulture/Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室,沈阳110161

出　　处：《沈阳农业大学学报》2018年第6期756-762,共7页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金青年项目(31801848);中国博士后科学基金项目(2018M631877);国家现代农业产业技术体系建设专项资金项目(CARS-25);辽宁省重大科技攻关项目(2011215003);沈阳市农业科技攻关专项项目(F11-092-3-00);沈阳市科技计划项目(17-143-3-00);辽宁省教育厅重点实验室项目(LZ2015064)

摘　　要：蛋白质的磷酸化是一种重要的蛋白质翻译后修饰方式。蛋白质的磷酸化与去磷酸化在植物气孔运动过程中起着关键的调节作用。目前,保卫细胞磷酸化蛋白质组学的主要研究内容包括鉴定磷酸化蛋白、定位磷酸化位点、定量磷酸化水平,进而揭示磷酸化和去磷酸化在植物气孔运动过程中所起的生物学功能。Open Stomata 1 (OST1)/SnRK2.6是蔗糖非酵解型蛋白激酶SnRK2(sucrose non-fermenting receptor kinase)家族的成员,具有典型的丝氨酸/苏氨酸蛋白激酶保守域,并主要在保卫细胞中表达。当植物处于正常生长环境时,ABA的受体PYR/PYL/RCAR不能作用于蛋白磷酸酶2C (PP2Cs,protein phosphatase 2Cs),PP2Cs通过与OST1互作抑制OST1的活性;在逆境胁迫下,PP2Cs解除对OST1蛋白激酶的抑制,随后OST1蛋白激酶启动对下游信号组分的调控作用并引起气孔运动。通过综述磷酸化修饰的定性和定量分析方法,以及蛋白质磷酸化修饰在保卫细胞应对非生物胁迫中的作用机制,提出了保卫细胞磷酸化蛋白组学领域目前存在的挑战和研究前景,旨在为深入了解保卫细胞应答非生物胁迫的气孔运动机制提供参考和新方向。Protein phosphorylation modification is recognized as the most important post-translational modification.Protein phosphorylation and dephosphorylation in guard cells under abiotic stress play essential roles in stomatal movement under stress conditions.Recently,the main research contents of phosphorylation proteomics in guard cells include identification of phosphorylated proteins,localization of phosphorylation sites,quantification of phosphorylation levels,and revealing the biological functions of phosphorylation and dephosphorylation in stomata movement.Open Stomata 1(OST1)/SnRK2.6,a major SnRK2-type protein kinase,has a typical conserved domain of Ser/Thr protein kinase,and it mainly expresses in guard cells.ABA receptor(PYR/PYL/RCAR)can't interact with Protein Phosphatase 2Cs(PP2Cs)under normal condition,and inhibit the activity of SnRK2.6(OST1)through interact with it.Under stress conditions,protein phosphatase type 2C relieves the inhibition of protein kinase OST1,which activates the regulation of the downstream components and induces stomatal movement.This review summarized the qualitative and quantitative analysis of phosphorylation modification,and mechanism of protein phosphorylation modification in protecting cells in response to abiotic stress.In addition,we also proposed provided some proposals for further research on guard cell phosphoprotomics.Our study aims are to provide a reference and indicate new directions for the mechanism study of stomata movement in response to abiotic stress.

关 键 词：磷酸化 保卫细胞 气孔运动 信号传导 非生物胁迫 

分 类 号：S625.2[农业科学—园艺学]