黑鲷幼鱼力竭运动后代谢酶活性的恢复水平  

Recovery level of metabolic enzymes in juvenile black sea bream (Sparus macrocephlus) after exhaustive exercise

作　　者：李丹丹 陈丕茂[1,2] 朱爱意 袁华荣 陈文静[1,2] 龙鑫玲 王文杰 LI Dandan;CHEN Pimao;ZHU Aiyi;YUAN Huarong;CHEN Wenjing;LONG Xinling;WANG Wenjie(Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment,Ministry of Agriculture and Rural Affairs;Key Laboratory of Marine Ranching Technology,Chinese Academy of Fishery Sciences;Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;Guangdong Engineering Technology Research Center of Marine Recreational Fishery;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;Zhejing Ocean University,Zhoushan 316022,China;Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院南海水产研究所,农业农村部南海渔业资源环境科学观测实验站,中国水产科学研究院海洋牧场技术重点实验室广东省渔业生态环境重点实验室,广东省海洋休闲渔业工程技术研究中心,广东广州510300 [2]浙江海洋大学,浙江舟山316022 [3]上海海洋大学,上海201306

出　　处：《南方水产科学》2018年第6期59-65,共7页South China Fisheries Science

基　　金：公益性行业(农业)科研专项经费项目(201003068);国家科技支撑计划项目(2012BAD18B02);中国水产科学研究院基本科研业务费专项资金项目(2016HY-ZD01);广东省2017年中央海洋经济物种增殖放流效果检验(2017-C01);深圳市科技计划项目(JSGG20141015154342147)

摘　　要：为探讨鱼类力竭运动后代谢水平的恢复情况,以黑鲷(Sparus macrocephlus)为实验对象,用手持续逆流追赶黑鲷幼鱼使其做力竭运动。结果显示,全长8.9～9.9 cm的黑鲷幼鱼力竭运动后,不同恢复时间血清中的乳酸(LD)和血糖(GLU)浓度,以及谷草转氨酶(AST)、谷丙转氨酶(ALT)、琥珀脱氢酶(SDH)、乳酸脱氢酶(LDH)、丙酮酸激酶(PK)和己糖激酶(HK)活性均有极显著变化(P<0.01)。力竭运动后第0小时,血清中的LD和GLU浓度及AST、ALT、SDH、LDH、PK、HK活性均显著升高(P<0.05);力竭运动后第4小时,血清中的LD浓度基本恢复到对照组水平(P>0.05);力竭运动后第16小时,血清中的SDH、HK、PK、AST活性恢复至对照组水平(P>0.05),但血清中的GLU浓度及LDH、ALT活性水平仍低于对照组(P<0.05),这表明黑鲷幼鱼力竭运动后第0到第4小时处于应激反应中,其无氧代谢水平得到短时提高。从血乳酸恢复水平上看,黑鲷幼鱼在首次力竭运动后4 h可再次进行力竭运动,但血糖检测结果显示此后无氧代谢耐力降低。Taking black sea bream(Sparus macrocephlus)as object,we investigated its recovery level of immune and metabolic enzymes in serum after exhaustive exercise.Results show significant change in the LD and GLU concentrations as well as the activities of AST,ALT,SDH,LDH,PK and HK for juvenile black sea bream(length of 8.9-9.9 cm)with different recovery time after exhaustive exercise(P<0.01).At 0^th hour after exhaustive exercise,the activities of LD,GLU,AST,ALT,SDH,LDH,PK and HK in-creased significantly(P<0.05).At 4^th hour after exhaustive exercise,the LD concentration in the serum almost recovered to the control group's level(P>0.05).At 16^th hour,the activities of SDH,HK,PK and AST recovered to the control group's level(P>0.05)while the GLU concentration and ALT activity were still lower than the control group's level(P<0.05).It is revealed that exhaustive exercise enhanced the oxidation resistance and aerobic metabolism of juvenile black sea bream within 4-hour stress response of postexercise,and the fish could do exhaustive exercise again after 4 h,but the blood glucose test shows that the anaerobic metabolism tolerance decreased thereafter.

关 键 词：黑鲷 代谢酶活性 力竭运动 恢复水平 

分 类 号：S917.4[农业科学—水产科学]