DNA发夹结构自组装信号放大策略应用的研究进展  

Research Progress in Application of Signal Amplification Strategy of Hairpin Structured DNA Self-assembly

作　　者：俞蓓 陈时建[2] 徐天雄 占忠旭 赖卫华[1] 许恒毅[1] YU Bei;CHEN Shi-jian;XU Tian-xiong;ZHAN Zhong-xu;LAI Wei-hua;XU Heng-yi(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang330047,China)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学科学技术学院,江西南昌330029

出　　处：《分析测试学报》2018年第12期1514-1520,共7页Journal of Instrumental Analysis

基　　金：江西省青年科学家(井冈之星)培养对象项目(2014BCB23004);江西省食品药品监督管理局科技计划(2015SP13)

摘　　要：DNA发夹结构自组装因具有无酶参与、等温以及识别序列能力强等优点,在生物分子和金属离子检测方面展现了良好的发展前景。该文梳理了DNA发夹结构自组装信号放大策略的类型,综述了近年来该策略在致病菌、核酸肿瘤标记物、蛋白质、无机金属离子,以及生物小分子检测中应用的研究进展,并对其未来发展趋势进行了展望,旨在为基于DNA发夹结构自组装检测生物分子提供一定的参考。Hairpin structured DNA self-assembly is widely used in the detection on biomolecules as it possesses the advantages of enzyme-free,isothermal and high recognition rate,etc.In this paper,the types of DNA self-assembly and its application in the detection of foodborne pathogens,tumor marker,protein,inorganic metal and small biomolecules are briefly summarized,and its future developments are also discussed so as to provide a reference for the study of biomolecules based on hairpin structured DNA self-assembly.

关 键 词：DNA结构自组装 分析检测 研究进展 

分 类 号：O629.7[理学—有机化学] G353.11[理学—化学]