香龙血树组织培养研究  

Study on Tissue Culture of Dracaena fragrans

作　　者：漆丽萍[1] 马剑[1] 李剑美[1] 李援龙 强继业[1] QI Li-ping;MA Jian;LI Jian-mei(Puer University,Puer,Yunnan 665000;Kunming Jiabin Commerce Limited Liabilit Company,Kunming,Yunnan 650000)

机构地区：[1]普洱学院,云南普洱665000 [2]昆明嘉滨商贸有限责任公司,云南昆明650000

出　　处：《安徽农业科学》2018年第34期71-74,共4页Journal of Anhui Agricultural Sciences

基　　金：云南省教育厅科学研究基金项目(2017zzx049);普洱学院高层次人才科研启动经费项目(K2015033)

摘　　要：[目的]通过香龙血树组织培养技术研究,建立香龙血树再生繁殖体系。[方法]以茎段作为外植体,探索最适灭菌时间以及诱导培养基及增殖培养基最适6-BA和NAA的浓度组合。[结果]灭菌最适宜时间9 min;以MS作为基本培养基,诱导培养最适的6-BA和NAA浓度组合:2.0 mg/L 6-BA+0.20 mg/L NAA易诱导长出丛芽,5.0 mg/L 6-BA+0.20 mg/L NAA和5.0 mg/L 6-BA+0.50 mg/L NAA易诱导形成愈伤组织;以MS作为基本培养基,增殖培养最适6-BA和NAA浓度组合:3.0 mg/L 6-BA+0.10 mg/L NAA。[结论]利用带腋芽茎段作为组织培养的外植体可迅速建立香龙血树离体快繁体系。[Objective]The regeneration and reproduction system of Dracaena fragrans was established via tissue culture.[Method]The optimal sterilization time and the concentration combination of6-BA and NAA for induction and proliferation media were determined using stem segments as explants.[Result]The optimum sterilization time was9minutes.For the MS basic medium,the optimal concentration combinations of6-BA and NAA for clumpy buds induction,callus formation and proliferation culture were2.0mg/L6-BA+0.20mg/L NAA,5.0mg/L6-BA+0.20mg/L NAA and5.0mg/L6-BA+0.50mg/L NAA,and3.0mg/L6-BA and0.10mg/L NAA,respectively.[Conclusion]The propagation system of D.fragrans in vitro could be rapidly established by using stem segments with axillary bud as explants for tissue culture.

关 键 词：组织培养 香龙血树 带腋芽茎段 快速繁殖 

分 类 号：Q943.1[生物学—植物学]