甘薯黑斑病菌快速大量产孢方法研究  

Study on Methods for Rapid Sporulation of Ceratocystis fimbriata

作　　者：王波[1] 黄忠勤[1] 丁震乾[1] 常勇[1] 周涧楠 苏在兴 周兴根[1] WANG Bo;HUANG Zhong-qin;DING Zhen-qian(Xuzhou Academy of Agricultural Sciences,Xuzhou,Jiangsu 221131)

机构地区：[1]江苏徐淮地区徐州农业科学研究所,江苏徐州221131

出　　处：《安徽农业科学》2018年第34期127-129,147,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立甘薯黑斑病菌快速大量产孢体系,为甘薯黑斑病品种抗性鉴定以及药剂生物测定奠定基础。[方法]采用3种方法制备甘薯黑斑病菌甘薯长喙壳菌(Ceratocystis fimbriata Ellis et Halsted)孢子悬浮液,研究甘薯黑斑病菌快速大量产孢方法。[结果]洗涤PDA和洗涤薯片培养5～6 d后镜检孢子悬浮液,一个视野内孢子数分别约为93、95个。利用PS培养液培养1、2、4 d后镜检孢子数分别为211、136、137个。[结论]采用PS液体培养基在特定培养条件下摇培1 d即可获得大量高浓度孢子悬浮液,极大地缩短了黑斑病菌产孢时间,显著提高了产孢量,且不易污染。[Objective]To establish rapid sporulation system for Ceratocystis fimbriata,and provide an important foundation for resistant cultivar identification and pesticide bioassay of Ceratocystis fimbriata.[Method]Three methods were used for sporulation of C.fimbriata to study methods for rapid sporulation of C.fimbriata.[Result]5-6days after cultured,in one field of view there were93and95spores in the control group1and the control group2,respectively.However,the numbers of spores were211,136and137after1day,2days and4days cultured in PS medium,respectively.[Conclusion]A large amount of high concentration spores suspension can be obtained by shaking culture1day in PS liquid medium under a specific culture condition,which can greatly shorten the sporulation time of C.fimbriata,also it was not easy to be polluted.

关 键 词：甘薯黑斑病 孢子悬浮液 制备方法 

分 类 号：S435.311[农业科学—农业昆虫与害虫防治]