全反式维甲酸通过TGF-β1/SMAD4信号通路对宫腔粘连细胞模型纤维化的影响  

Effect of ATRA in the fibrosis of intrauterine adhesions cells model through TGF-β1/SMAD4 signaling pathway

作　　者：刘丽敏[1] 史文娟[1] 何芳[1] 胡海燕[1] LIU Limin;SHI Wenjuan;HE Fang;HU Haiyan(Department of Gynecology,Shenzhen Maternity and Child Healthcare Hospital,Shenzhen 518000,China)

机构地区：[1]深圳市妇幼保健院妇科,广东深圳518000

出　　处：《实用医学杂志》2018年第22期3680-3684,共5页The Journal of Practical Medicine

基　　金：深圳市科技创新基金资助项目(编号:JCYJ20170307091700193)

摘　　要：目的评估全反式维甲酸(ATRA)对宫腔粘连细胞模型纤维化的影响。方法培养原代人子宫内膜基质细胞(HESCs),ICC法鉴定细胞;用0、2.5、5、10和20 ng/mL TGF-β1诱导HESCs 48 h,qRT-PCR检测COL1A1、α-SMA的表达;以10 ng/mL TGF-β1诱导HESCs 48 h,继而0、0.1、1和10μmol/mL ATRA作用48 h,qRT-PCR、WB检测COL1A1、α-SMA、及SMAD4的表达。结果间质细胞标记物Vimentin阳性,上皮细胞标志物CK-18阴性;与0 ng/mL TGF-β1组相比,其他组COL1A1和α-SMA的表达升高,以10 ng/mL组表达最高(P <0.01);与0μmol/mL ATRA组相比,1、10μmol/mL ATRA组COL1A1、α-SMA和SMAD4的表达下降,以1μmol/mL组下降最明显(P <0.01)。结论 TGF-β1诱导HESCs发生肌成纤维细胞样改变,构建宫腔粘连细胞模型,ATRA通过TGF-β1/SMAD4信号通路改善其纤维化。Objective Evaluate the effect of ATRA in the fibrosis of intrauterine adhesions cells model.Methods HESCs were cultured by the primary way and identified by ICC.HESCs were treated with different doses of 0,2.5,5,10 and 20 ng/mL TGF-β1 for 48 h,and then detected expressions of COL1A1 andα-SMA by qRT-PCR.After treatment of 10 ng/mL TGF-β1 for 48 h,HESCs were treated with ATRA at the different doses of 0,0.1,1 and 10μmol/mL for 48 h.The mRNA and protein expressions of COL1A1,α-SMA and SMAD4 were detect.ed by qRT-PCR and WB.Results The results of ICC showed that Vimentin of the mesenchymal cell marker was positive and CK-18 was negative.Compared with 0 ng/mL TGF-β1 group,the expressions of COL1A1 andα-SMA in others groups were increased,with the highest expression in 10 ng/mL TGF-β1 group(P<0.01).The mRNA and protein expressions of COL1A1,α-SMA and SMAD4 were decreased in 1 and 10μmol/mL ATRA groups com.pared with 0μmol/mL ATRA group.Conclusion TGF-β1 can induce the fibrosis of HESCs.ATRA alleviates the fibrosis of HESCs through TGF-β1/smad4 signaling pathway.

关 键 词：子宫内膜基质细胞 宫腔粘连 转化生长因子P1 纤维化 ATRA 

分 类 号：R711.74[医药卫生—妇产科学]