饲料添加多维对花鲈生长、非特异性免疫指标及消化酶活性的影响  

Effects of multi-vitamins on the growth performance, non-specific immunity indices and digestive enzymatic activity of Lateolabrax japonicus

作　　者：蒋鹏达 李文静[1,2] 黎中宝 方秀[3] 汪晴 Jiang Pengda;Li Wenjing;Li Zhongbao;Fang Xiu;Wang Qing(Fisheries College, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China;Fujian Provincial Key Laboratory of Breeding of Lateolabrax japonicas, Ningde 355200, China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省海洋渔业资源与生态环境重点实验室,福建厦门361021 [3]福建省花鲈育种企业重点实验室,福建宁德355200

出　　处：《海洋学报》2018年第12期40-48,共9页

基　　金：福建省科技计划项目(2015N0010);厦门市科技项目(3502Z20143017);福建省自然科学基金项目(2013J01139)

摘　　要：本试验旨在研究饲料中不同水平的多维含量对花鲈(Lateolabrax japonicus)生长性能、非特异性免疫指标及消化酶活性的影响。以初始体质量为(38.54±0.05)g的花鲈为试验对象,随机分为6组(每组3个重复,每个重复30尾鱼),基础饲料中分别添加不同水平(0.5%、1%、2%、3%、4%、5%)的多维,在室内循环养殖系统中饲喂28 d。结果表明:多维添加量对花鲈的体成分、存活率、肝指数、饲料系数和肥满度均没有显著影响(P>0.05);当多维添加量为2%时,花鲈的增重率(WGR)和特定生长率(SGR)均最大,与多维添加量为2%相比较,随着多维添加量继续增多,花鲈的WGR和SGR显著降低(P<0.05);当多维添加量为5%时,花鲈的肠蛋白酶活性较0.5%和1%添加量组显著提高(P<0.05),而多维添加量为0.5%时,花鲈的肠淀粉酶活性显著高于其余任意一组(P<0.05),各组之间肠脂肪酶活性无显著差异(P>0.05);与多维添加量为0.5%相比,多维添加量为5%时,能够显著提高花鲈血清中超氧化物歧化酶、碱性磷酸酶活性以及血清中总蛋白含量(P<0.05),而血清溶菌酶活性没有显著变化(P>0.05)。结果表明:饲料中添加多维含量为5%时,能够明显提高花鲈的非特异性免疫指标及肠道蛋白酶活性,而在含量为2%时,能够明显提高花鲈的生长性能。This report aimed to investigate the effects of basal diets by adding different dosages(0.5%,1%,2%,3%,4%,5%)of multi-vitamins on the growth performance,non-specific immunity indices and digestive enzymatic activity of Lateolabrax japonicus.The fishes with initial body mass of(38.54±0.05)g were fed28days in indoor close-recirculating culture systems,and divided into six groups,each group contained30individuals and set in triplicate.The results showed that the dosage of multi-vitamins had no significant influence to the body composition,survival rate,hepatosmatic index and feed conversion ratio(P>0.05)of L.japonicus;the weight gain rate(WGR)and specific growth rate(SGR)reached highest at2%diet;with the increase of multi-vitamin dosages,WGR and SGR decreased significantly(P<0.05)compared to2%diet.The5%diet improved the intestinal protease activity,while the intestine amylase(AMS)activity of the control group was significantly higher than any other groups(P<0.05),but there was no significant influences on intestinal LPS activity(P>0.05).Intestinal total protein(TP)content reached maximum at3%diet with significant difference(P<0.05).The5%diet improved the activity of total superoxide dismutase(T-SOD),alkaline phosphatase(AKP)and TP content in serum significantly(P<0.05),while serum LSZ activity had no significant change(P<0.05).In conclusion,the feed by adding5%multi-vitamins significantly improved the non-specific immunity indices of L.japonicus and the intestinal protease activity,while the feed supplemented with2%multi-vitamins obviously promoted the growth of L.japonicus.

关 键 词：花鲈 多维 生长性能 消化酶 免疫指标 

分 类 号：P917.4[天文地球—自然地理学]