γδT细胞及其产生的IL-17A在急性川崎病患儿中的改变及临床意义  

Changes and clinical significance of γδT cells and Interleukin-17A produced in children with acute Kawasaki disease

作　　者：刘志强[1] 彭宇[1] 邹峥[1] 柯江维[1] LIU Zhiqiang;PENG Yu;ZOU Zheng;KE Jiangwei(Jiangxi Provincial Children's Hospital Nanchang Jiangxi 330006)

机构地区：[1]江西省儿童医院,江西南昌330006

出　　处：《实验与检验医学》2018年第6期815-817,1023,共4页Experimental and Laboratory Medicine

基　　金：江西省自然科学基金资助项目(20122BAB205085)

摘　　要：目的观察急性川崎病(KD)患儿外周血γδT细胞及IL-17A的变化,探讨产生IL-17A的γδT细胞在KD发病中改变及意义。方法分离人外周血单个核细胞(PBMCs),豆蔻酰佛波醇乙酯刺激后采用流式细胞仪检测γδT细胞和γδT17细胞占CD3+T细胞的百分比,同时采用ELISA测定血清IL-17A和TGF-β1水平,并比较两组间γδT、γδT17、IL-17A和TGF-β1的变化。结果相对于健康对照组,病例组患儿外周血γδT、γδT17、IL-17A和TGF-β1均明显增高[7.09±0.63 vs 11.24±2.31,1.25±0.53 vs 3.68±1.70,(4.41±1.09)ng/l vs (11.19±3.48)ng/L,(6.93±1.37)ng/ml vs (9.31±1.84)ng/ml](P<0.05)。结论γδT细胞在急性KD患儿高度活化,并且通过产生高水平细胞因子IL-17A介导了KD的炎症反应。Objective To observe the changes ofγδT cells and IL-17A in children with acute Kawasaki disease(KD),and to investigate the changes and significance of IL-17-producingγδT cells in the pathogenesis of KD.Methods The human peripheral blood mononuclear cells(PBMCs)were separated,and the percentage ofγδT cells andγδT17 cells in CD3+T cells was detected by flow cytometry after stimulation with PMA.At the same time,serum IL-17A and TGF-β1 levels were measured by using ELISA.The changes ofγδT,γδT17,IL-17A and TGF-β1 between the two groups were compared.Results Compared with the healthy control group,theγδT,γδT17,IL-17A and TGF-β1 in the peripheral blood of the case group were significantly increased[7.09±0.63 vs 11.24±2.31,1.25±0.53 vs 3.68±1.70,(4.41±1.09)ng/l vs(11.19±3.48)ng/L,(6.93±1.37)ng/ml vs(9.31±1.84)ng/ml](P<0.05).ConclusionsγδT cells are highly activated in children with acute KD,and may participate in the pathogenesis of KD by producing a high level of IL-17A.

关 键 词：ΓΔT细胞 细胞因子 儿童 川崎病 

分 类 号：R446.113[医药卫生—诊断学] R446.112[医药卫生—临床医学]