齐齐哈尔地区仔猪腹泻大肠杆菌血清型与毒力基因分析  

Analysis of the Virulence Genes and Serotype Test of Piglet Diarrhea Escherichia Coli in Qiqihar

作　　者：陈楠楠[1] 史同瑞[1] 尹珺伊[1] 侯美如[1] 王岩[1] 秦平伟[1] 刘宇[1] 朱战波[2] Chen Nannan;Shi Tongrui;Yin Junyi;Hou Meiru;Wang Yan;Qin Pingwei;Liu Yu;Zhu Zhanbo(Heilongjiang Institute of Veterinary Medicine Science,Qiqihar 161006;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University)

机构地区：[1]黑龙江省兽医科学研究所,齐齐哈尔161006 [2]黑龙江八一农垦大学动物科技学院

出　　处：《黑龙江八一农垦大学学报》2018年第6期19-23,共5页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省财政厅科研院所项目(sczzn2016-7)

摘　　要：为了解黑龙江省齐齐哈尔地区仔猪腹泻大肠杆菌的优势血清型和毒力基因流行情况,从齐齐哈尔地区40个养猪场的腹泻仔猪体内分离到100株致病性大肠杆菌,通过凝集试验和分子生物学检测方法进行血清型及毒力基因检测。血清型检测结果表明,100株大肠杆菌有49个分离株鉴定出血清型,其中O8、O9、O101、O147、O157、O64为优势血清型,占定型菌株的77.55%。毒力基因PCR测定结果表明,黏附素基因987P(50/71,70.42%)、肠毒素基因LT(65/71,91.54%)、强毒力岛FyuA(43/71,60.56%)为主要流行的毒力基因。研究揭示了齐齐哈尔地区仔猪腹泻大肠杆菌的优势血清型和主要携带的毒力基因,为临床防治大肠杆菌性仔猪腹泻提供了科学依据。To investigate the prevalence of piglet diarrhea Escherichia coli in Qiqihar,Heilongjiang province,100strains of pathogenic E.coli were isolated from diarrhea piglets in vivo in40pig farms which were detected by means of agglutination test and molecular biological detection techniques.The results showed that49were placed in serogroups among100isolates.The dominant serotypes were O8,O9,O101,O14,O157,O64,which accounted for77.55%of the identified strains.Virulence gene PCR determination results showed that the adhesion gene:987P(50/71,70.42%),LT(65/71,91.54%),FyuA(43/71,60.56%)were the major epidemic virulence genes.

关 键 词：齐齐哈尔地区 猪 致病性大肠杆菌 血清型 毒力基因 

分 类 号：S855.12[农业科学—临床兽医学]