拟南芥突变体jar1的鉴定及其MeJA感受性分析  

Identification and MeJA Sensitivity Analysis of Arabidopsis Mutant jar1

作　　者：乔菊香 杨红玉[2] 吴嘉 彭浈[2] 沈放 周丽娟[2] 王云月[1] Qiao Juxiang;Yang Hongyu;Wu Jia;Peng Zhen;Shen Fang;Zhou Lijuan;Wang Yunyue(Key Laboratory of Ministry of Education for Agricultural Biodiversity and P)est Management,College of Plant Protection, Yunnan Agricultural Lniversity,Kunming Yunnan 650201,China;College of Life Science and Technology,Kunming Lniversity,Kunming Yunnan 650214,China)

机构地区：[1]云南农业大学植物保护学院,生物多样性与病害控制教育部重点实验室,云南昆明650201 [2]昆明学院生命科学与技术系,云南昆明650214

出　　处：《西南林业大学学报（自然科学）》2018年第6期69-73,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31760078,31260062)资助。

摘　　要：为了研究JAR1 (At2g46370)基因在茉莉酸调控机制中的作用,从拟南芥生物资源中心获得JAR1基因的T-DNA插入突变体jar1 (SALK_030821),对突变体中T-DNA插入位点和突变纯合性进行检测,筛选出纯合的jar1突变体。通过qRT-PCR检测jar1突变体中JAR1基因的表达水平,并进行茉莉酸敏感性实验,观察jar1幼苗在含有茉莉酸甲酯的培养基的生长情况。结果表明:纯合突变体中JAR1基因的表达量明显下降,仅为野生型植株的41. 2%; jar1幼苗对茉莉酸甲酯的敏感性显著下降。1n order to study the role of JAR1(At2g46370)gene in the mechanism of JA signaling,a mutant jar1(SALK_030821)inserted by T-DNA was obtained from the Arabidopsis Biological Resource Center.The homozygous jar1 mutants were screened out after the position of T-DNA insertion were identified.The expression level of the JAR1 gene in jar1 mutants was analyzed by quantitative real-time qRT-PCR,JA sensitivity was detected,and the growth of the seedling in the medium containing MeJA was observed.The results show that the expression of JAR1 gene was dramatic reduction,it is about 41.2%of wild type plant.Comparing to the wild type,the sensibility of homozygous mutant jar1 seedling roots to MeJA was also decreased.

关 键 词：JAR1基因 jar1突变体 纯合体 MeJA感受性 

分 类 号：Q344.12[生物学—遗传学]