ThZFP1蛋白特异性结合的DNA顺式作用元件  

DNA Cis-acting Element Specifically Recognized by ThZFP1 Protein

作　　者：段肖肖 余振强 臧丹丹[1] 张丹丹[1] 陈韵如 王衷涵 牛亦龙 王玉成[1] Duan Xiaoxiao;Yu Zhenqiang;Zang Dandan;Zhang Danan;Chen Yunru;Wang Zhonghan;NiuYilong;Wang Yucheng(State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, P. R. China)

机构地区：[1]林木遗传育种国家重点实验室(东北林业大学),哈尔滨150040

出　　处：《东北林业大学学报》2018年第12期35-38,共4页Journal of Northeast Forestry University

基　　金：东北林业大学大学生创新训练项目(201610225008)

摘　　要：锌指蛋白(ZFP)是一类通过结合锌离子折叠成手指结构域的蛋白,在植物逆境胁迫中起重要作用。Th ZFP1需要结合一定的DNA序列来调控基因的表达,为了确定Th ZFP1结合的元件序列,利用自己开发的TFCentered Y1H技术,鉴定了Th ZFP1蛋白识别的核心序列,分别为DOF和ARR1AT。并进一步在植物体内验证了Th ZFP1蛋白与DOF和ARR1AT元件的互作。结果表明,Th ZFP1蛋白能够特异性地识别DOF和ARR1AT顺式作用元件来调控相关基因的表达。Zinc finger protein( ZFP) is a kind of protein which was folded into a finger structure by combining zinc ions,and plays an important role in plant osmotic stress. We used a transcription factor centered yeast one hybrid( TF-Centered Y1 H) to study the cis-acting element bound by Th ZFP1. The result showed that DOF and ARR1 AT motifs were specifically recognized by Th ZFP1 and proved by bombardment and GUS activity assays. Therefore,Th ZFP1 regulated the gene expression by binding with DOF and ARR1 AT motifs specifically.

关 键 词：TH ZFP1 DNA随机文库 蛋白质-DNA互作 基因枪 GUS酶活测定 

分 类 号：Q943[生物学—植物学]