川芎嗪通过抗氧化及抗炎作用改善脊髓损伤  

Antioxidation Effect and Antiinflammation Effect of Ligustrazine on Improving Spinal Cord Injury

作　　者：都芳涛[1] 方继峰[1] 李兴晶 乔培柳 李广义[1] DU Fangtao;FANG Jifeng;LI Xingjing;QIAO Peiliu;LI Guangyi(Liaocheng Second People's Hospital Affiliated to Shandong Medical University, Linqing 252600, China)

机构地区：[1]山东泰山医学院附属聊城市第二人民医院骨外科,山东临清252600

出　　处：《中医药学报》2018年第6期95-98,共4页Acta Chinese Medicine and Pharmacology

基　　金：山东省中医药科技发展计划项目(2017-495)

摘　　要：目的:探讨川芎嗪对脊髓损伤的修复作用及机理。方法:将90例不同程度的脊髓损伤患者按照随机盲法分成三组,分别为对照组、高剂量川芎嗪组和低剂量川芎嗪组,每组30人。对照组患者在治疗过程中静脉滴注250 m L 0. 9%氯化钠注射液,连续静滴14 d。高剂量川芎嗪组病例静脉滴注250 m L0. 9%氯化钠注射液+240 mg川芎嗪,连续静滴14 d。低剂量川芎嗪组病例静脉滴注250 m L 0. 9%氯化钠注射液+120 mg川芎嗪,连续静滴14 d。每个患者静脉滴注后进行腰椎穿刺抽取脑脊液进行实时定量PCR测定和促炎性细胞因子的生化检测。结果:与对照组相比,应用川芎嗪治疗的脊髓损伤患者脑脊液中TNF-α与IL-1β的mRNA表达降低,与低剂量组相比,高剂量组降低更加明显。另外,应用川芎嗪治疗的脊髓损伤患者脑脊液中Nrf2、GCLc与GCLm的mRNA表达上调,与低剂量组相比,高剂量组患者上调更加明显。结论:川芎嗪可能通过改善炎性反应及应激反应来促进脊髓损伤的康复。Objective:To investigate the effect and mechanism of ligustrazine on repairing spinal cord injury.Methods:90patients with different degrees of spinal cord injury were randomly divided into the control group(n=30),the high-dose ligustrazine group(n=30)and the low-dose ligustrazine group(n=30).The control group was given0.9%sodium chloride injection(250mL);on which basis,the ligustrazine groups were also injected with ligustrazine,which was240mg in the high-dose ligustrazine group and120mg in the low-dose ligustrazine group.The treatment was14days.Spinal tap was carried to extract cerebrospinal fluid(CSF)for real-time quantitative PCR test and for biochemical detection of pro-inflammatory cytokines after venous instillation in patients.Results:The mRNA expressions of TNF-αand IL-1βin CSF were significantly decreased in ligustrazine groups,compared to the control group;the decrease was more obvious in the high-dose group.The mRNA expressions of Nrf2,GCLc and GCLm in CSF were significantly up-regulated in ligustrazine groups;the increase was more significant in the high-dose group.Conclusion:Ligustrazine may promote the recovery of spinal cord injury by improving inflammatory reactions and stress responses.

关 键 词：脊髓损伤 川芎嗪 炎性反应 氧化应激 NRF2 GCLc GCLm 

分 类 号：R269[医药卫生—中西医结合]