高效液相色谱法同时测定栀芩清热合剂中三种成分的含量  

Determination of three components in zhiqin qingre mixture by HPLC

作　　者：郝乘仪 于蕾 昌盛 刘哲 HAO Chengyi;YU Lei;CHANG Sheng;LIU Zhe(College of Pharmacy,Jilin Medical University,Jilin City,Jilin Province,132013,China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2019年第1期4-6,共3页Journal of Jilin Medical University

基　　金：吉林市科技局课题(20165020)

摘　　要：目的建立栀芩清热合剂中京尼平苷酸、京尼平龙胆双糖苷、绿原酸含量测定方法。方法色谱柱为PLATSILTMODS色谱柱(250 mm×4. 6 mm,5μm);流动相为乙腈(A)-0. 1%磷酸水溶液(B);流速为1 m L/min;柱温在30℃。结果京尼平苷酸(240 nm)线性范围是0. 409 5～4. 914 0μg(R2=0. 999 4);京尼平龙胆双糖苷(240 nm)线性范围是0. 025 0～0. 300 0μg(R2=0. 990 9);绿原酸(327 nm)线性范围是0. 020 5～0. 246 0μg(R2=0. 990 7)。结论该方法准确可靠、精密度好,适用于栀芩清热合剂的质量控制。Objective To establish a method for the determination of geniposide acid,genipin gentian diglycoside and chlorogenic acid in zhiqin qingre mixture.Methods The chromatographic column was PLATSIL TM ODS column(250 mm×4,6 mm,5μm)Mobile phase was acetonitrile(A)-0.1%phosphoric acid solution(B).The flow rate is 1 mL/min.The temperature of chromatographic column was 30℃.Results The linear range of chlorogenic acid(240 nm)was 0.409 5~4.914 0μg(R 2=0.999 4),and the linear range of genipin gentian diglycoside(240 nm)was 0.025 0~0.300 0μg(R 2=0.990 9),while the linear range of hlorogenic acid(254 nm)was 0.020 5~0.246 0μg(R 2=0.990 7).Conclusion The method is accurate,reliable and precision,and is suitable for the quality control of zhiqin qingre mixture.

关 键 词：芩清热合剂 高效液相色谱法 京尼平苷酸 京尼平龙胆双糖苷 绿原酸 

分 类 号：R286[医药卫生—中药学]