通腑化痰法对急性脑出血大鼠肝细胞凋亡的影响及对应激性肝损害的保护作用  

Effect of Tongfu Huatan Method on Hepatocyte Apoptosis and Protection of Stress Liver Injury in Rats with Acute Cerebral Hemorrhage

作　　者：王威 马迪 杜泓森 侯季秋 黄乐曦 赵海滨 Wang Wei;Ma Di;Du Hongsen

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《中国中医急症》2018年第12期2095-2097,2106,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：中央高校基本科研业务费专项资金资助(2015-JYB-QNJSZX010)

摘　　要：目的观察通腑化痰法中药对急性脑出血大鼠肝细胞凋亡的影响及对应激性肝损害的保护作用。方法选用健康雄性SD大鼠,随机分成正常对照组、脑出血模型组、通腑化痰组及葡醛内酯组。采用大脑注射胶原酶法建立大鼠脑出血模型,在造模后各时相点对各组大鼠肝功能水平检测,对肝组织HE染色结果进行观测,同时采用TUNEL法检测各组大鼠肝细胞凋亡指数。结果通腑化痰组较脑出血模型组血清谷丙转氨酶(ALT)、谷草转氨酶(GOT)水平显著降低(P <0.05或P <0.01)。通腑化痰组大鼠肝组织病理检测显示病变明显减轻,肝组织结构基本正常,炎性细胞浸润现象较少。脑出血模型组各时相点均可见较多凋亡细胞,48 h可见凋亡细胞达高峰,经通腑化痰法干预后,各时相点TUNEL阳性细胞明显减少,肝细胞凋亡数比较有明显差异(P <0.05)。结论急性脑出血大鼠应激性肝损害,引起大鼠肝功能损伤、肝细胞凋亡数及肝组织病理学明显改变,经过中药通腑化痰法干预后,对大鼠肝细胞凋亡产生明显抑制作用,并有效改善肝组织病理学结构及肝功能水平,为临床防治急性脑出血应激性肝损害的中医药治疗提供实验依据。Objective:To investigate the changes of hepatocyte apoptosis and liver histopathology in rats with acute cerebral hemorrhage induced by stress liver injury,and clarify the effect of Tongfu Huatan method on hepatocyte apoptosis and the protective effect on stress liver injury.Methods:Healthy male SD rats were randomly divided into the control group,the model group,Tongfu Huatan group and Glucurolactone group.The rat model of cerebral hemorrhage was established by injecting collagenase into the brain.The liver function was measured at each time point after the model was established.HE staining results of liver tissue were observed.At the same time,the apoptotic index of liver cells was measured with Tunel method.Results:The levels of serum ALT and GOT in the Tongfu Huatan group were significantly lower than those in the model group(P<0.05or P<0.01).Pathological examination of liver tissue of Tongfu Huatan group showed that the pathological changes were significantly alleviated,that liver tissue structure was basically normal,and that inflammatory cell infiltration was less.There were more apoptotic cells at each time point in the model group,and they reached the peak in48hours.After the intervention of Tongfu Huatan method,Tunel positive cells were significantly reduced at each time point,and the number of hepatocyte apoptosis was significantly different(P<0.05).Conclusion:Stress liver injury in rats with acute cerebral hemorrhage can cause significant changes in liver function,hepatocyte apoptosis and liver histopathology.The intervention of Tongfu Huatan method can inhibit hepatocyte apoptosis,improve the pathological structure of liver tissue and the level of liver function,and provide experimental basis for the clinical treatment of acute cerebral hemorrhage stress liver injury.

关 键 词：急性脑出血 应激性肝损害 肝细胞凋亡 通腑化痰法 

分 类 号：R285.5[医药卫生—中药学]