复方丹参注射液对重症急性胰腺炎肺损伤大鼠CYLD/NF-κB信号的影响  

Effect of Compound Danshen Injection on CYLD/NF-κB Signal in Rats with Severe Acute Pancreatitis-acute Lung Injury

作　　者：马丹[1] 包先丽 叶霜 陶然[2] Ma Dan;Bao Xianli;Ye Shuang(Hepingli Hospital of Beijing,Beijing 100013,China)

机构地区：[1]北京市和平里医院,北京100013 [2]北京安贞医院,北京100029

出　　处：《中国中医急症》2018年第12期2098-2102,2119,共6页Journal of Emergency in Traditional Chinese Medicine

基　　金：北京市科学技术委员会科研计划项目(Z151100002113008)

摘　　要：目的探究复方丹参注射液对重症急性胰腺炎(SAP)肺损伤(ALI)大鼠肺组织的改善作用,并探究其可能的作用机制。方法将SD大鼠分为假手术组、模型组、复方丹参注射液(SM)低、中、高剂量组、地塞米松组,每组12只大鼠,HE染色观察胰腺、肺组织损伤情况;测量腹水量、肺组织干、湿质量;测定肺泡灌洗液(BAL)中中性粒细胞(PMN)数量以及动脉血中血气指标;蛋白免疫印迹(WB)、免疫组化法分析CYLD/NF-κB通路蛋白表达;酶联免疫吸附法检测BAL中炎性细胞因子水平。结果与假手术组比较,模型组大鼠胰腺、肺组织病理评分、腹水量、胰腺、肺组织W/D、PMN、PaCO2、p-p65、p-IκBα蛋白表达、TNF-α、IL-1β、IL-6、MDA升高,PaO2、OI、CYLD蛋白表达、SOD水平降低(P <0.05)。与模型组相比,SM组、地塞米松组胰腺、肺组织病理评分、腹水量、胰腺、肺组织W/D、PMN、PaCO2、p-p65、p-IκBα蛋白表达、TNF-α、IL-1β、IL-6、MDA均降低,PaO2、OI、CYLD蛋白表达、SOD水平均升高(P <0.05)。结论复方丹参注射液可缓解SAP-ALI大鼠炎症损伤,可能与激活肺组织CYLD、抑制/NF-κB信号通路激活有关。Objective:To explore the improvement effect of Compound Danshen Injection on lung tissues of rats with severe acute pancreatitis(SAP)-acute lung injury(ALI),and to explore its possible mechanism.Methods:SD rats were divided into the sham operation group(sham),SAP-ALI group,low dose,middle dose and high dose Compound Danshen Injection(SM)groups,and Dexamethasone group(DXM),with12rats in each.The damages of pancreas and lung tissues was observed by HE staining;the amount of ascites,lung tissue dry and wet weights,the number of neutrophils(PMN)in the bronchoalveolar lavage fluid(BAL)and blood gas index in arterial blood were measured;Western blot(WB)and immunohistochemistry were used to analyze the protein expression of CYLD/NF-κB pathway,enzyme-linked immunosorbent assay to detect the levels of inflammatory cytokines in BAL.Results:Compared with the sham group,all indexes of SAP-ALI group were improved(P<0.05).Compared with the SAP-ALI group,the indexes of SM group and DXM group were improved(P<0.05).Conclusion:Compound Danshen Injection can alleviate inflammatory injury of SAP-ALI rats,which may be related to activation of CYLD in lung tissue and inhibition of activation of NF-κB signaling pathway.

关 键 词：复方丹参注射液 重症急性胰腺炎肺损伤 圆柱瘤蛋白 核因子转录蛋白 

分 类 号：R285.5[医药卫生—中药学]