中国水仙愈伤组织诱导及植株再生研究  

Study on the Callus Induction and Plant Regeneration of Chinese Narcissus

作　　者：蔡月琴 车蕾 陆銮眉 邹金美 CAI Yueqin;CHE Lei;LU Luanmei;ZOU Jinmei(School of Biological Sciences and Biotechnology,Minnan Normal University,Zhangzhou,Fujian 363000,China)

机构地区：[1]闽南师范大学生物科学与技术学院,福建漳州363000

出　　处：《闽南师范大学学报（自然科学版）》2018年第4期61-67,共7页Journal of Minnan Normal University：Natural Science

基　　金：福建省科技厅重点项目(2014N0028);闽南师范大学科研项目(MK201706)

摘　　要：以中国水仙鳞茎为试材,进行中国水仙愈伤组织诱导及其分化的研究.结果表明:1)2%次氯酸钠20 min较适合水仙花花苞的消毒处理,水仙鳞茎的消毒处理以0. 1%升汞12 min灭菌效果较好,以内层鳞片存活率最高,外层鳞片次之;2)在MS+6-BA2. 0 mg·L-1+2,4-D 0. 5 mg·L-1为愈伤诱导及继代的培养基上,子房壁愈伤诱导率最高,为77. 8%,但继代未见再分化,外层鳞片愈伤诱导率次之,外层鳞片与带鳞片的鳞茎盘诱导的愈伤均能继代发育成小鳞茎;3)以培养基MS+6-BA 2. 0 mg·L-1+NAA 0. 5 mg·L-1较适合外层鳞片愈伤组织的再分化,鳞茎分化率达64. 4%;4)在培养基1/2 MS+IBA1. 0 mg·L-1上,水仙鳞茎苗生根效果较好,生根率达86. 7%,移栽成活率高.It studied the callus induction and differentiation of various explants such as bulb,filament of Chinese narcissus(Narcissus tazetta var.chinensis).The results showed that:1)The best sterilization method for unopened flowers of Narcissus was to soak them into 70%alcohol for 60 s plus 2% NaClO for 20 min with the survival rate of 51.1%and for bulb of Narcissus was to soak them into 70% alcohol for 60 s plus 0.1% HgCl2 for 12 min after pretreatment as the inner bulb scale with the survival rate of 73.3%.2)Callus induction and differentiation of all these explants were induced on the MS medium containing 2 mg·L^-1 6-BA and 0.5 mg·L^-1 2,4-D,the callus-inducing frequency of the ovary wall was much higher than that of others,but without redifferentiation in the subculture.Both the callus induced from outer scales and basal plate with segments could differentiate and develop into the bulbils.3)The more suitable medium for the differentiation of outer scales callus was MS+2.0 mg·L^-1 6-BA+0.5 mg·L^-1 NAA,in which the frequency of bulblet regeneration was up to 64.4%.4)The optimum medium for rooting of the bulblet was 1/2 MS supplement with 1.0 mg·L^-1 IBA,which of the rooting rate reached to 86.7%and had high survival rate after transplanting.

关 键 词：中国水仙 愈伤组织 脱分化 再分化 

分 类 号：S632.1[农业科学—蔬菜学] Q943.1[农业科学—园艺学]