Effects of Salt Stress on Stipa breviflora Seedlings and Antioxidant Isozymes  

作　　者：Jing WANG Hongzhou ZI Huangwei LI Yanxia NING Ling LUO 

机构地区：[1]College of Biological Science and Engineering,North Minzu University

出　　处：《Agricultural Biotechnology》2018年第6期63-67,共5页农业生物技术（英文版）

基　　金：Supported by Introduced Personnel Research Start-up Fund of North Minzu University(4400302550);National Natural Science Foundation of China(31600202)

摘　　要：Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol/L NaC l,to investigate the effects of salt stress on the physiological phenotype and antioxidant enzyme activity of S.breviflora seedlings.The results showed the germination rate,root length and fresh weight of seedlings were all influenced by salinity.Germination rate,root length and fresh weight of seedlings were significantly inhibited by 100,150 and50 mmol/L NaC l,and were 62%,1.30 cm,0.299 g,respectively.With NaC l content increasing,the trend of superoxide dismutase( SOD) and peroxidase( POD) activity was rising at first and then falling,and the activity of SOD and POD reached the maximum at 150 and 100 mmol/L,48.6 and 6 766 U/g,respectively.The activity of catalase( CAT) continued to increase and reached the maximum at 150 mmol/L,299 U/g.The isozyme zymogram showed that SOD 2-4,POD 1-2,and CAT 1-2 were positively correlated with salinity tolerance of S.breviflora seedlings.Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol/L NaC l,to investigate the effects of salt stress on the physiological phenotype and antioxidant enzyme activity of S.breviflora seedlings.The results showed the germination rate,root length and fresh weight of seedlings were all influenced by salinity.Germination rate,root length and fresh weight of seedlings were significantly inhibited by 100,150 and50 mmol/L NaC l,and were 62%,1.30 cm,0.299 g,respectively.With NaC l content increasing,the trend of superoxide dismutase( SOD) and peroxidase( POD) activity was rising at first and then falling,and the activity of SOD and POD reached the maximum at 150 and 100 mmol/L,48.6 and 6 766 U/g,respectively.The activity of catalase( CAT) continued to increase and reached the maximum at 150 mmol/L,299 U/g.The isozyme zymogram showed that SOD 2-4,POD 1-2,and CAT 1-2 were positively correlated with salinity tolerance of S.breviflora seedlings.

关 键 词：STIPA breviflora SALT stress SOD POD CAT 

分 类 号：S54[农业科学—作物学]