金针菇寡肽转运蛋白编码基因fvopt1与fvopt2的鉴定与表达分析  

Identification and Expression Analysis of Two Oligopeptide Transporter Encoding Genes fvopt1 and fvopt2 from Flammulina velutipes

作　　者：韩星 魏仲阳 张晔琳 黄蓉梅 黄千慧 谢宝贵[2] HAN Xing;WEI Zhongyang;ZHANG Yeline;HUANG Rongmei;HUANG Qianhui;XIE Baogui(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Mycological Research Center,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002

出　　处：《食用菌学报》2018年第4期17-23,137,共8页Acta Edulis Fungi

基　　金：国家重点基础研究发展计划项目(2014CB138302)

摘　　要：基于金针菇(Flammulina velutipes)的基因组和转录组数据,通过本地BLAST方法,获得2个寡肽转运蛋白编码基因,命名为fvopt1和fvopt2,并对其结构、编码蛋白的基本性质、系统进化与表达模式进行分析。结果表明:fvopt1和fvopt2分别含有13和18个外显子,编码蛋白FVOPT1与FVOPT2各含774和772个氨基酸,相对分子质量为86120.25与86192.24,等电点为7.46与7.92,且均无信号肽。保守结构域分析与系统进化分析结果表明,FVOPT1与FVOPT2为寡肽转运蛋白家族成员。实时荧光定量PCR结果表明:在菌丝阶段,fvopt1在无氮培养基中的表达量高于其在含氮培养基中的表达量;在含氮培养基中,fvopt1在整个子实体发育阶段(原基、菌柄和菌盖)中表达量上调,fvopt2只在原基中高表达,推测fvopt1和fvopt2可能与子实体和原基的发育有关。Using the local BLAST, two genes encoding oligopeptide transporter, fvoptl and fvopt2, were identified from the genome and transcriptome sequences of Flammulina velutipes. Gene structures, basic characteristics of deduced proteins, phylogenetic evolution and expression patterns of both genes were analyzed. The results showed that fvoptl and fvopt2 contained 13 and 18 exons, respectively; and their deduced proteins FVOPT1 and FVOPT2 consisted of 774 and 772 amino acids, respectively. Molecular weights of FVOPT1 and FVOPT2 were 86120.25 and 86192.24; their isoelectric points were 7.46 and 7.92,respectively. Neither protein contained a signal peptide. Conserved domain analysis and phylogenetic analysis revealed that FVOPT1 and FVOPT2 belonged to the oligopeptide transporter superfamily. Results of real time quantitative PCR indicated that the expression level of fvoptl during the mycelium stage was higher on nitrogen-free medium than on nitrogen-containing medium. When cultivated on nitrogen-containing medium,the expression level of fvoptl was up-regulated during the fruiting body development stage, including primordium, stipe and pileus. On the other hand, fvopt2 was only highly expressed in primordium.Therefore, fvopt1 and fvopt2 were hypothesized to be functionally important to the development of fruiting body and primordium, respectively.

关 键 词：氮源 短肽 底物偏好性 OPT家族 信号肽 

分 类 号：S646.15[农业科学—蔬菜学]