不同培养条件对茯苓产孢量的影响  

Effect of Culture Condition on Sporulation of Wolfiporia cocos

作　　者：曾桂萍[1,2] 李忠 张文龙[3] 赵致[1,2] ZENG Guiping;LI Zhong;ZHANG Wenlong;ZHAO Zhi(Guizhou University,Guiyang Guizhou,550025,China;Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guiyang Guizhou,550025,China;Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou550002,China)

机构地区：[1]贵州大学,贵州贵阳550025 [2]贵州省药用植物繁育与种植重点实验室,贵州贵阳550025 [3]贵阳中医学院,贵州贵阳550002

出　　处：《食用菌学报》2018年第4期43-46,138,共5页Acta Edulis Fungi

基　　金：贵州省药用植物繁育与种植人才基地建设项目{黔人领发[2013]15号};贵州省科技计划项目{黔科合基础[2018]1042};贵州省中药材现代农业产业技术体系建设项目(GZCYTX2015-0202)

摘　　要：通过比较培养基组成、培养温度、培养基初始pH、光照对茯苓(Wolfiporia cocos)产孢的影响,探讨产孢量较大的培养基组成和培养条件。结果表明:装有60 mL茯苓PDA培养基(200 g马铃薯,20 g葡萄糖,1 g KH_2PO_4,0.5 g MgSO_4·7H_2O,10 mg维生素B_1,18 g琼脂,pH自然,加蒸馏水补足至1000 mL)的350 mL组培瓶,培养基初始pH为6.5,28℃暗培养7 d,12 h光暗交替培养为最优产孢条件,在此条件下,茯苓培养38 d产孢量为(8.12±0.11)×10~9个/瓶。In order to explore optimal medium and culture conditions for sporulation of Wolfiporia cocos,effect of medium component, temperature, initial pH after autoclave and light condition were studied. The results showed that the optimal conditions for sporulation were PDA medium（350 mL with 60 mL medium）composed of 200 g/L potato, 20 g/L glucose, 1 g/LKH2 PO4, 0.5 g/L MgSO4·7H2O, 10 mg/L VB1, and18 g/L agar with natural initial pH at 6.5, cultivation at 28 ℃ in the dark for 7 days followed with a light cycle of 12 h/12 h（irradiation/in the dark）. Spore yield under the optimal condition was（8.12 ± 0.11） x 109 per flask on day 38.

关 键 词：茯苓 有性孢子 产孢量 

分 类 号：S567.32[农业科学—中草药栽培]