大黄鱼烂鳃病病原菌的分离鉴定及药敏分析  

Isolation,identification and antibiotic sensitivity analysis of bacterial pathogen from Larimichthys crocea with gill-rot disease

作　　者：游剑涛[1] YOU Jiantao(Xiamen Ocean Vocational College,Xiamen361102,China)

机构地区：[1]厦门海洋职业技术学院,福建厦门361102

出　　处：《渔业研究》2018年第6期425-433,共9页Journal of Fisheries Research

摘　　要：2013年宁德网箱养殖大黄鱼出现烂鳃病症状,病鱼鳃丝溃烂、软骨裸露;从濒死的患烂鳃病大黄鱼(Larimichthys crocea)鳃部分离得到1株优势菌,经人工感染试验证实为引起烂鳃的致病菌。生理生化特征显示,致病菌在TCBS培养基上形成表面光滑、湿润,边缘整齐的黄色菌落,为革兰氏阴性弧杆状,具有运动性,在含6%氯化钠的培养基中生长,对V-P、阿拉伯糖、靛基质、枸橼酸盐等显阴性;对半乳糖、触酶、淀粉酶等显阳性。结合形态学、生理生化指标和16S rRNA序列分析,确定该病原菌为弧菌Vibrio ponticus。药敏试验表明该病原菌对氨苄西林、多粘菌素B、复方新诺明、诺氟沙星、哌拉西林、庆大霉素、头孢哌酮、氧氟沙星等16种药物敏感。研究结果为弧菌引发的大黄鱼病害防治提供参考依据。In 2013,the large yellow croaker(Larimichthys crocea)cultured in net cage at Ningde was diseased.The sick fish appeared symptoms of gill filaments ulcers and gill rakers bare.One dominant bacterial strain,named as Lc-2013-G1,was isolated from the gill of sick L.crocea.The strain Lc-2013-G1 was confirmed to be a bacterial pathogen of L.crocea by artificial infection.Physiological and biochemical characteristics showed that the pathogen Lc-2013-G1 formed a smooth,moist,marginally yellow colony on the TCBS medium,which was Gram-negative and arc-shaped rod.The strain Lc-2013-G1 had motility and grew in a medium containing 6%sodium chloride.The indicators of V-P,arabinose,sputum matrix and citrate were negative to Lc-2013-G1,while the indicators of galactose,catalase and amylase were positive to Lc-2013-G1.The pathogen Lc-2013-G1 was determined to be Vibrio ponticus based on morphological and physiological detection,biochemical tests and 16S rRNA gene analysis.Antibiotic sensitivity analysis indicated that the pathogen Lc-2013-G1 was sensitive to Ampicillin,Polymyxin B,Sulfamethoxazole,Norfloxacin,Piperacillin,Gentamycin,Cefoperazone,Ofloxacin,and so on.The results provided a reference for the prevention and treatment of large yellow croaker disease caused by Vibrios.

关 键 词：大黄鱼(Larimichthys crocea) VIBRIO ponticus 分离鉴定 药敏分析 

分 类 号：S947.9[农业科学—水产养殖]