投喂频率对工厂化循环水养殖珍珠龙胆石斑鱼生长和血浆抗应激酶活力的影响  

The effect of feeding frequency on the growth and plasma anti-stress enzyme activities of hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) in industrial recirculating aquaculture systems

作　　者：仇登高[1,2] 温凭 林琪[1,2] 朱志煌 吴水清[1,2] 刘银华 陈新明 邱峰岩[4] QIU Denggao;WEN Ping;LIN Qi;ZHU Zhihuang;WU Shuiqing;LIU Yinhua;CHEN Xinming;QIU Fengyan(Fisheries Research Institute of Fujian,Xiamen361013,China;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Xiamen361013,China;Freshwater Fisheries Research Institute of Fujian,Fuzhou350002,China;Xiamen Xiaodeng Aquatic Science and Technology Co.Ltd.,Xiamen361104,China)

机构地区：[1]福建省水产研究所,福建厦门361013 [2]福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013 [3]福建省淡水水产研究所,福建福州350002 [4]厦门小嶝水产科技有限公司,福建厦门361104

出　　处：《渔业研究》2018年第6期441-448,共8页Journal of Fisheries Research

基　　金：福建省省属公益类科研院所基本科研专项(2016R1003-8;2016R1003-13;2017R1003-2);福建省海洋与渔业结构调整专项(珍珠龙胆石斑鱼高效循环水养殖技术优化与应用);福建省海洋高新产业发展专项[闽海洋高新(2014)21号];福建省海洋生物增养殖与高值化利用重点实验室开放课题基金(2016fjscq05);厦门南方海洋研究中心项目(14GZP75NF39)

摘　　要：在水温(29. 96±0. 40)℃条件下,选用人工配合饲料饲养初始体质量为(297. 79±59. 94) g的珍珠龙胆石斑鱼,经过60 d的养殖试验,研究了不同投喂频率1次/d、2次/d、1次/2d对工厂化循环水养殖系统中珍珠龙胆石斑鱼生长和血浆抗应激酶活力的影响。结果表明:投喂频率为1次/2d的成活率(99. 39±0. 01)%,均显著高于投喂频率为1次/d的(96. 75±0. 66)%和2次/d的(97. 08±0. 66)%(P <0. 05);各试验组间的特定生长率(SGR=1. 05～1. 19%/d)、肥满度(CF=1. 63～1. 77)、相对增质量(RWG=187. 65%～205. 04%)、日增质量(DWG=4. 33～5. 00 g/d)、体质量变异系数(SV=14. 50～18. 30)、日摄食率(FI=1. 07～1. 24%/d)、饲料转化效率(FCE=81. 77%～87. 38%)及饲料系数(FCR=1. 15～1. 25)均无显著影响(P>0. 05);在血浆抗应激酶活力方面,投喂频率对珍珠龙胆石斑鱼血浆中的碱性磷酸酶(AKP=8. 07～8. 26金氏单位/100 mL)、过氧化氢酶(CAT=1. 92～2. 52 U/mL)及超氧化物歧化酶(SOD=23. 39～24. 34 U/mL)也均无显著影响(P>0. 05)。在本试验条件下,可以采用1次/2d的投喂频率来提高工厂化循环水养殖珍珠龙胆石斑鱼的成活率。The experiment was conducted for 60 days in order to determine the effects of different feeding frequencies(1 time per day,2 times per day,and once every 2 days)on the growth and plasma anti-stress enzyme activities of the hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)with initial weight of(297.79±59.94)g at water temperature of(29.96±0.40)℃,reared with artificial formulated in industrial recirculating aquaculture systems.The results showed that the survival rate of the fish fed once every 2 days(99.39±0.01)%was significantly higher than those of the fish fed once per day(96.75±0.66)%and twice a day(97.08±0.66)%(P<0.05).There were not significant effects on the specific growth rate(SGR=1.05~1.19%/d),condition factor(CF=1.63~1.77),relative weight gain(RWG=187.65%~205.04%),daily weight gain(DWG=4.33~5.00 g/d),coefficient of size variation(SV=14.50~18.30),food intake(FI=1.07~1.24%/d),food conversion efficiency(FCE=81.77%~87.38%)and food conversion ratio(FCR=1.15~1.25)among the three groups(P>0.05).The plasma anti-stress enzyme activities(i.e.AKP=8.07~8.26 King/100mL,CAT=1.92~2.52 U/mL and SOD=23.39~24.34 U/mL)of the fish was not significantly different in all treatments(P>0.05).Under the experimental conditions,the feeding frequency of once every two days could be used to improve the survival rate of the hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)in industrial recirculating aquaculture systems.

关 键 词：珍珠龙胆石斑鱼 投喂频率 生长 血浆抗应激酶活力 工厂化循环水养殖 

分 类 号：S967[农业科学—水产养殖]