祛风通络方对阿霉素肾病大鼠超微结构及足细胞相关蛋白CD2AP和整合素α3β1表达的影响  

Effect of Qufeng Tongluo Recipe on Ultrastructure and Expression of Podocyte CD2 Associated Protein and Integrin α3β1 in Adriamycin Nephropathy Rats

作　　者：全建峰 杨宁 孙万森[2] 马巧亚[2] QUAN Jian-feng;YANG Ning;SUN Wan-sen;MA Qiao-ya

机构地区：[1]陕西中医药大学附属医院,咸阳712000 [2]西安交通大学第二附属医院,咸阳710004

出　　处：《河北中医药学报》2018年第6期5-8,共4页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：国家自然科学基金资助项目:No.30672697/H2708;国家自然基金面上项目:No.81072757;陕西省科技厅资助项目:No.2013K-12-07-10

摘　　要：目的:研究祛风通络方对阿霉素肾病(AN)大鼠肾小球超微结构变化及肾小球足细胞上相关蛋白CD2associated protein (CD2AP)和整合素α3β1表达的影响,探讨祛风通络方治疗肾小球病的作用及机理。方法:60只雄性清洁级SD健康大鼠随机分为空白对照组和模型组,采用一次性尾静脉注射阿霉素的方法,建立大鼠AN模型,造模成功后再将模型动物随机分为模型组、祛风通络组和激素组,分别给予祛风通络方和强的松干预。透射电镜观察足细胞超微结构,免疫组化染色检测大鼠肾脏CD2AP和整合素α3β1蛋白表达,并分析CD2AP和整合素α3β1与足细胞超微结构关系。结果:造模成功时较空白对照组,模型组大鼠超微结构显示:增宽、收缩、甚至消失。干预结束时与模型组相比较:⑴各干预组超微结构病变均有不同程度改善,足突结构较模型组清晰;⑵祛风通络组和激素组CD2AP和整合素α3β1蛋白表达明显上调(P <0. 01),两者之间未见明显差异。结论:⑴AN大鼠肾小球足细胞结构与功能均发生了改变,表现为足突融合甚至消失,肾小球足细胞相关蛋白CD2AP和整合素α3β1蛋白表达下降。⑵祛风通络方通过修复足细胞超微结构,有效改善足细胞上CD2AP和整合素α3β1蛋白表达,从而达到保护肾小球足细胞分子屏障及治疗肾小球病的作用。Objective:To study the effect of Qufeng Tongluo Recipe on the ultrastructural changes of glomerulus and the expression of CD2associated protein(CD2AP)and integrinα3β1in glomerular podocytes of rats with adriamycin nephropathy(AN),and to explore the effect and mechanism of Qufeng Tongluo Recipe on glomerulopathy.Methods:Sixty male SD healthy rats of clean grade were randomly divided into blank control group and model group.AN model rats were established by one-off caudal vein injection of adriamycin.After successful modeling,the model animals were randomly divided into model group,Qufeng Tongluo group and hormone group,and were given Qufeng Tongluo recipe and prednisone intervention respectively.The ultrastructure of podocytes was observed by transmission electron microscopy,the expression of CD2AP and integrinα3β1in rat kidneys was detected by immunohistochemical staining,and the relationship between CD2AP and integrinα3β1and the ultrastructure of podocytes was analyzed.Results:When the model was successfully established,the ultrastructure of rats in the model group showed widening,contraction and even disappearance.At the end of the intervention,compared with the model group,the ultrastructural changes were improved in different degrees in each intervention group,and the structure of foot process was clearer than that in the model group;the expression of CD2AP and integrinα3β1in Qufeng Tongluo group and hormone group were significantly increased(P<0.01),but there was no significant difference between the two groups.Conclusion:(1)The structure and function of glomerular podocytes in AN rats have changed,which shows foot process fusion or even disappearance,and the expression of CD2AP and integrin a3|3l decreased.(2)Qufeng Tongluo Recipe can effectively improve the expression of CD2AP and integrinα3β1in podocytes by repairing the ultrastructure of podocytes,thus protecting the molecular barrier of glomerular podocytes and treating glomerulopathy.

关 键 词：祛风通络方 阿霉素肾病 CD2AP 整合素Α3Β1 超微结构 肾小球病 

分 类 号：R285.5[医药卫生—中药学]