分枝杆菌病噬菌体33D基因组学特征及抗结核潜力  

Genetic information of mycobacteriophage 33D and the potentials in control of tuberculosis

作　　者：邬亭亭[1,2] 魏强 郭述良[2] 刘平 罗永艾[2] WU Tingting;WEI Qiang;GUO Shuliang;LIU Ping;LUO Yongai(Department of Respiratory Medicine,The Third People's Hospital of Chengdu,Chengdu 610031,China;Department of Respiratrry and Critical Care Medicine,The First Af filiated Hospital of Chongqing Medical University,Chongqing 400016,China;Department of Internal Medicine,Chengdu Tianfu New District Peoples Hospital,Chengdu 610213,China;Department of Respiratory Medicine,The People's Hospital of Changshou District,Chongqing 401220,China)

机构地区：[1]成都市第三人民医院呼吸内科,四川成都610031 [2]重庆医科大学附属第一医院呼吸与危重症医学科,重庆400016 [3]成都市天府新区人民医院内二科,四川成都610213 [4]重庆市长寿区人民医院呼吸内科,重庆401220

出　　处：《西部医学》2018年第12期1728-1733,1737,共7页Medical Journal of West China

基　　金：国家自然科学基金面上项目(81570010);四川省卫计委科研课题(17PJ381)

摘　　要：目的研究分枝杆菌噬菌体33D的基因组特性并探索其抗结核治疗的潜力。方法通过鸟枪法完成33D全基因组测序;使用DNAStar、TRF、Promoter predictions、tRNAscan-SE、Glimmer、BLAST软件分别对33D基因组进行分析。结果噬菌体33D基因组为线性双链DNA,全长50036bp;含有84个推定基因,17个已知功能的基因。其中噬菌体DNA复制相关基因:gene6、7、8、9和gene 14;裂解酶基因:gene 47和gene 48。结论噬菌体33D为烈性噬菌体,具有抗结核潜力,未发现致病基因,可作为"鸡尾酒疗法"的候选噬菌体。Objective To explore the genetic information of phage 33D and its anti-tuberculosis potentials.Methods The genome of phage 33D were sequenced.The gene annotations of those genomes were completed by DNAStar,TRF,Promoter predictions,tRNAscan-SE,Glimmer and BLAST.Results The genome of phage 33D was a dsDNA sequence of 50036 bp.The genome of phage 33D encodes 84 gene,17 gene functions had been identified by BLAST comparison.We propose that gene47 and gene 48 could encode lyases.We proposed that gene6,7,8,9 and 14 were related to DNA Replication.Conclusion 33D is lytic phage which have potentials in control of tuberculosis and have not virulence gene.Phage 33D could be used in phage cocktail therapy.

关 键 词：分枝杆菌噬菌体33D 基因组 结核分枝杆菌 

分 类 号：R378.911[医药卫生—病原生物学]