花生红衣水提物对小鼠的镇静催眠作用  

Experimental study of peanut red skin water extract on sedative and hypnotic effects in mice

作　　者：孙涛 林娜 李军 齐汝霞 林丽文 张鹏 SUN Tao;LIN Na;LI Jun;QI Ruxia;LIN Liwen;ZHANG Peng(College of Basic Medicine,Jining Medical University,Jining 272067,China)

机构地区：[1]济宁医学院基础医学院,济宁272067

出　　处：《济宁医学院学报》2018年第6期388-392,共5页Journal of Jining Medical University

基　　金：济宁市医药卫生科技项目(2013JNWK52);济宁医学院青年基金项目(JYQ2011KM011);山东省医药卫生科技发展计划项目(2017WS773)

摘　　要：目的探讨花生红衣水提物对小鼠的镇静催眠作用及机制。方法采用小鼠自主活动测试仪观察记录小鼠自主活动,协同戊巴比妥钠致小鼠睡眠实验观察花生红衣水提物对小鼠的镇静催眠作用,酶联免疫吸附法(ELISA)测定小鼠脑组织中γ-氨基丁酸(γ-aminobutyric acid,GABA)含量。结果自主活动实验表明,与空白对照组相比,低剂量组花生红衣水提物可抑制小鼠自主活动能力(P <0. 05);协同戊巴比妥催眠实验结果显示,与空白对照组相比,低剂量组花生红衣水提物显著增加阈下剂量戊巴比妥钠小鼠的入睡率(P <0. 05),能缩短阈上剂量戊巴比妥钠小鼠的入睡潜伏期(P <0. 05),延长睡眠持续时间(P <0. 05); ELISA测定结果显示,低剂量组花生红衣水提物可显著升高小鼠脑组织中GABA含量(P <0. 05)。花生红衣水提物各剂量组之间不存在量效关系。结论花生红衣水提物有一定的镇静催眠作用,其作用机制可能与提高小鼠脑内GABA水平有关。Objective To investigate the Sedative and hypnotic effects of peanut red skin extract and explore the potential mechanism.Methods Sedative-hypnotic experiments were conducted to observe the spontaneous activity in mice and its sleep effect on the superthreshold and subthreshold doses of sodium pentobarbital.ELISA method was employed to detect the contents of GABA in brain of mice.Results Low doses of peanut red skin water extract could significantly reduced the number of spontaneous activity in mice(P<0.05),and showed synergic effect on pentobarbital sodium of subthreshold dose by increased the sleep rate of mice(P<0.05),decreased the sleep latency time and prolonged the sleeping time with superthreshold dose of pentobarbital sodium(P<0.05).The ELISA results showed that low doses of peanut red skin water extract could increased the content of GABA in brain of mice significantly(P<0.05).There was no dose-effect relationship among the three dose groups of peanut red skin water extract.Conclusion The peanut red water extract has a function of sedative and hypnotic and its mechanism may be related to the increase of GABA level in brain of mice.

关 键 词：花生红衣水提物 镇静催眠 小鼠 

分 类 号：R963[医药卫生—微生物与生化药学] R971.3[医药卫生—药理学]