荧光染色法和KOH湿片法、真菌培养法在皮肤浅部真菌感染诊断中的应用比较  

Comparasion of the fluorescent staining method and KOH wet method,fungal culturing method in detecting skin superficial fungal infection

作　　者：杜鹏 黄嘉怡 沈守星 魏秋姣 刘小凤 DU Peng;HUANG Jiayi;SHEN Shouxing;WEI Qiujiao;LIU Xiaofeng(Zhuhai Center for Chronic Disease Control,Zhuhai 519000,Guangdong,China)

机构地区：[1]珠海市慢性病防治中心,广东珠海519000

出　　处：《中国麻风皮肤病杂志》2018年第12期712-714,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:评估荧光染色法在皮肤浅部真菌感染诊断中的价值。方法:收集我院皮肤门诊疑似皮肤浅部真菌感染标本,同时采用荧光染色法、KOH湿片法和真菌培养法进行检测,比较检测结果。结果:在236例标本中荧光染色法、KOH湿片法、真菌培养法总阳性率分别为90.6%、67.4%、60.2%,在179例皮屑标本中阳性率分别为92.1%、69.3%、64.2%,52例甲真菌病标本中阳性率分别为84.6%、57.7%、44.2%,荧光染色法与其他两种方法相比,差异均有统计学意义(均P<0.01)。5例毛发标本中阳性率分别为100%、100%、80%,三种方法阳性率无统计学差异(均P>0.05)。结论:荧光染色法简单有效,可以降低假阴性率。Objective:To evaluate the effects of fluorescent staining method in detecting skin superficial fungal infection.Methods:Suspected patients with fungal infection were collected in the department of dermatology,and all the samples were detected by fluorescent staining method,KOH wet method and fungal culturing method.Results:The total positivity rate of 236 samples by the fluorescent staining method,KOH wet method and fungal culturing method were 90.6%,67.4%and 60.2%respectively.The positivity rate of 179 lesions by the fluorescent staining method,KOH wet method and fungal culturing method were 92.1%,69.3%,64.2%.The positivity rate of 52 nails were 84.6%,57.7%and 44.2%respectively..There were significant differences of above positivity rate between fluorescent staining method and KOH wet method or fungal culturing method(P s<0.01).The positivity rate of 5 hear samples by the fluorescent staining method,KOH wet method and fungal culturing method were 100%,100%and 80%respectively.There was no significant difference of hear positivity rate among three methods(P>0.05).Conclusion:Fluorescent staining is a simple and effective method,and it can reduce false negativity rate.

关 键 词：荧光染色 KOH 真菌培养 皮肤浅部真菌感染 

分 类 号：R756[医药卫生—皮肤病学与性病学]