基于SSR的高粱光敏特性分析  

Analysis on the Photosensitivity of Sorghum Based on SSR

作　　者：牛皓[1,2,3] 平俊爱 张福耀[1,2,3] 吕鑫 杜志宏[1,2,3] 李慧明 王玉斌[1,2,3] NIU Hao;PING Jun-ai;ZHANG Fu-yao(Institute of Sorghum,Shanxi Academy of Agricultural Sciences, Jinzhong,Shanxi 030600;Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Jinzhong,Shanxi 030600;Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan,Shanxi 030031)

机构地区：[1]山西省农业科学院高粱研究所,山西晋中030600 [2]高粱遗传与种质创新山西省重点实验室,山西晋中030600 [3]农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《安徽农业科学》2018年第35期97-99,共3页Journal of Anhui Agricultural Sciences

基　　金：高粱遗传育种与种质创新山西省重点实验室青年基金项目(GLS16-3);山西省农业科学院优势课题组自选项目(YYS1706);现代农业产业技术体系建设专项(CARS-06-01-01);酿造专用高粱育种及利用山西省科技创新重点团队项目(2014131015)

摘　　要：[目的]探明控制高粱抽穗期基因的遗传机理,为高粱品种选育提供新思路新方法。[方法]利用已知抽穗性状的3个高粱材料晋粱五、Ma、IS722,对144对SSR引物进行聚丙烯酰胺凝胶电泳检测,分析差异,筛选光敏感特异性引物。[结果]有4对引物产生特异性条带,分别为xtxp217、xtxp12、xtxp15、xtxp4,且片段大小在100 bp左右,其中xtxp217位于10号染色体、xtxp12位于4号染色体、xtxp15位于5号染色体、xtxp4位于2号染色体。[结论]该研究为后续对高粱杂交种进行光敏鉴定提供了基础。[Objective]To elucidate the genetic mechanism of genes controlling heading date of sorghum and provide new ideas and methods for sorghum variety breeding.[Method]Three pairs of sorghum materials,Jinliang No.5,Ma and IS722,were used to detect144pairs of SSR primers by polyacrylamide gel electrophoresis.The difference was analyzed and the photosensitive specific primers were screened.[Result]Four pairs of primers produced specific bands,which were xtxp217,xtxp12,xtxp15and xtxp4,and the fragment size was about100bp.Xtxp217is on chromosome10,xtxp12is on chromosome4,xtxp15is on chromosome5and xtxp4is on chromosome2.[Conclusion]The study provides a basis for subsequent photosensitive identification of sorghum hybrids.

关 键 词：高粱 光敏感 SSR 

分 类 号：S514[农业科学—作物学]