圣地红景天组织培养繁殖研究  

Study on Tissue Culture and Reproduction of Rhodiola sacra

作　　者：周志疆[1,2] 陈明亮 杨佩娟 ZHOU Zhi-jiang;CHEN Ming-liang;YANG Pei-juan(Shanghai Academy of Agricultural Sciences,Shanghai 201106;Jiangsu Harvest Land Seed Industry Development Limited Company,Yancheng,Jiangsu 224100)

机构地区：[1]上海市农业科学院,上海201106 [2]江苏丰收大地种业发展有限公司,江苏盐城224100

出　　处：《安徽农业科学》2018年第35期103-104,170,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省大丰区科学技术局本级科技计划项目(DFJH201816)

摘　　要：[目的]建立圣地红景天组织培养繁殖体系。[方法]以圣地红景天叶片为外植体,接种在附加不同激素组合的MS培养基诱导愈伤组织和不定芽的形成,同时考察不同培养基对不定根形成的影响,优化种植基质、提高种苗移栽成活率。[结果]叶片在MS+30 g/L蔗糖+1.0 g/L圣地红景天叶片+0.5 mg/L 6-BA+0.05 mg/L NAA培养基上的愈伤组织诱导率高达98%,随后的不定芽分化率为67%。壮苗生根培养基为1/2 MS,将种苗栽植于泥炭∶珍珠岩∶蛭石=2∶1∶1的基质上,其移栽成活率为85%。[结论]利用该体系培育的圣地红景天种苗长势优良,移栽成活率高。[Objective]The research aimed to establish the tissue culture and rapid propagation of Rhodiola sacra.[Method]Using the leaves of Rhodiola sacra as material,callus and adventitious bud induction were studied on1/2MS medium supplemented with different hormone combinations,at the same time,the effect of different culture medium on rooting was investigated.In order to improve the survival rate in transplantation,composition of planting substrate was optimized.[Result]The leaves of Rhodiola sacra could induce callus when cultured in the MS medium supplemented with30g/L sugar,1.0g/L leaves of Rhodiola sacra,0.5mg/L6-BA and0.05mg/L NAA;the highest induction rate could reach98%,and the rate of adventitious bud differentiation was67%with continuous cultured.Rhodiola sacra were easy to root on the1/2MS medium.Seedlings were planted in substrate,the substrate composed with peat,perlite and vermiculite in a ratio of2∶1∶1,the survival percent of transplantation could reach85%.[Conclusion]With this culture system,the seedlings of Rhodiola sacra have excellent growth and high survival rate.

关 键 词：激素配比 圣地红景天 组织培养 移栽 

分 类 号：S567.23[农业科学—中草药栽培]