MDCK细胞微载体培养条件优化研究  

Optimization of Culture Conditions for MDCK Cell Micro-carriers

作　　者：马启财 武文莉 马富龙 潘和平[1] MA Qi-cai;WU Wen-li;MA Fu-long;PAN He-ping(College of Life Science and Engineering,North University for Nationalities,Lanzhou 730030)

机构地区：[1]西北民族大学生命科学与工程学院,兰州730030

出　　处：《生物技术通报》2018年第12期90-95,共6页Biotechnology Bulletin

基　　金：甘肃省重点研发计划-农业类项目(17YF1NA067)

摘　　要：研究细胞接种量、搅拌转速和微载体浓度对MDCK细胞微载体培养时的影响,以合理优化MDCK细胞微载体培养最大增殖时期的最优条件,对疫苗和病毒分离具有重要意义,以期达到在疫苗和病毒分离领域提高生产效率。采用微载体培养MDCK细胞,在不同搅拌转速、微载体浓度和细胞接种量进行培养,每隔24 h取样计数,确定最优的培养条件。结果表明,细胞接种量在20个/球、搅拌转速45 r/min和载体浓度在2 g/L时,MDCK细胞的增殖较快,细胞密度较大,细胞的密度最大可达15.6×106个/mL,适合MDCK细胞增殖生长。Studying the effect of cell inoculation quantity,stirring speed and microcarrier concentration on the MDCK cells in microcarrier culture and optimizing the conditions of culturing MDCK cells micro-carrier during the age of the largest proliferation is of great significance for the separation of vaccine from virus,aiming at increasing the efficiency in separating vaccine from viruses.In this study,MDCK cells were cultured with a micro-vector and cultured at different stirring speed,concentration of the micro-vector,and amount of cell inoculation,and the optimal culture conditions were determined by sampling and counting every24h.The results showed that MDCK cells proliferation was faster and cell density was larger up to16.5x106cells/mL while cell inoculation in20balls,stirring speed in45r/min,and carrier concentration in2g/L,which is suitable for MDCK cells proliferation and growth.

关 键 词：MDCK细胞 细胞量 微载体培养 

分 类 号：Q813[生物学—生物工程]