不同免疫程序接种PRRS疫苗对仔猪血清抗体水平的影响  

Effect of PRRS Vaccines with Different Immunization Schedules on Serum Antibody Levels in Piglets

作　　者：丁尊俄 赵双成 张双翔[4] 周碧君[1,2] 肖焕星 欧伟业 王开功[1,2] 秦文学 刘昱 胡兴义 张海[1,2] 李如举 陈勇 DING Zun-e;ZHAO Shuang-cheng;ZHANG Shuang-xiang;ZHOU Bi-jun;XIAO Huan-xing;OU Wei-ye;WANG Kai-gong;QIN Wen-xue;LIU Yu;HU Xing-yi;ZHANG Hai;LI Ru-ju;CHEN Yong(College of Animal Science,Guizhou University,Guiyang,Guizhou,550025,China;Animal Disease Laboratory of Guizhou Province,Guiyang,Guizhou,550025,China;Shanghai Hile Bio-technology Co.,Ltd,Shanghai,201403,China;Animal Disease Prevention and Control Center in Guizhou Province,Guiyang,Guizhou,550001,China;Guizhou Ruite New Agriculture and Animal Husbandry Science and Technology Co.,Ltd,Guiyang,Guizhou,550001,China;Guizhou Kaili Hongda Animal Husbandry Development Co.,Ltd,Kaili,Guizhou,556000,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025 [3]上海海利生物技术股份有限公司,上海201403 [4]贵州省动物疫病预防控制中心,贵州贵阳550001 [5]贵州瑞特新科农牧科技有限公司,贵州贵阳550001 [6]贵州省凯里市宏大牧业发展有限公司,贵州凯里556000

出　　处：《动物医学进展》2018年第12期15-19,共5页Progress In Veterinary Medicine

基　　金：贵州省科学技术厅农业攻关项目(黔科合NY[2015]3009-1号);贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队(黔科合人才团队[2015]4016号)

摘　　要：为了解仔猪以不同免疫程序接种猪繁殖与呼吸综合征(PRRS)疫苗后产生的血清抗体水平,在规模化猪场筛选猪繁殖与呼吸综合征病毒(PRRSV)和抗体均为阴性的仔猪40头,随机分为4组。其中试验A组、试验B组和试验C组均为14d首次接种经典PRRS弱毒疫苗,试验A组在35d二次免疫高致病性PRRSV灭活疫苗,试验B组在35d二次接种经典PRRS弱毒疫苗,试验C组在35d注射生理盐水,对照组D组在14d、35d均注射生理盐水。在仔猪免疫接种后0、7、14、21、28、35、42、49、56和63d分别采血,用N-ELISA和G-ELISA两种试剂盒检测PRRSV特异性抗体。结果显示,N-ELISA抗体水平A组与B组差异不显著(P>0.5),而G-ELISA抗体水平在免疫后35d、56d、63dA组显著高于B组(P<0.5)。表明14d首次接种经典PRRSV弱毒疫苗和35d二次接种高致病性PRRSV灭活疫苗的免疫效果优于仅单独一次或两次接种PRRSV弱毒疫苗效果。In order to explore the serum antibody levels of piglets inoculated with porcine reproductive and respiratory syndrome(PRRS)vaccine in different immunization programs,40piglets without PRRSV antigen and antibody were selected and randomly divided into four groups.Groups A,B and C were immunized with the classical attenuated PRRS vaccine for the first time at14-day-old age,group A was boosted with highly pathogenic inactivated vaccine at35-day-old age;group B was boosted with the classical attenuated vaccine at35-day-old age;group C was given with normal saline at35-day-old age;and control group D was given with normal saline at14-and35-day-old ages.Then blood samples were collected at0,7,14,21,28,35,42,49,56and63d,respectively.After immunization,the PRRSV specific antibodies were examined by N-ELISA and G-ELISA kits.The results showed that N-ELISA antibody levels in group A and group B were not significant(P>0.5);G-ELISA antibody levels in group A were higher than that in group B at35,56and63d(P<0.5).As indicated above,the immune effect of classical PRRSV attenuated vaccine at14d and highly pathogenic inactivated vaccine for the second time at35d were better than that of only once or twice classical attenuated vaccine immunization.

关 键 词：猪繁殖与呼吸综合征 弱毒疫苗 灭活疫苗 抗体 免疫效果 

分 类 号：S852.4[农业科学—基础兽医学] S852.659.6[农业科学—兽医学]