黄芪粗提物对四氯化碳诱导的小鼠急性肝损伤的保护作用  

Protective Effect of Astragalus Crude Extracts on CCl_4-induced Acute Liver Injury in Mice

作　　者：鲍家卉 王雪慧 逄淑伟 王莹[1] 刘馨宇[1] 张雪梅[1] BAO Jia-hui;WANG Xue-hui;PANG Shu-wei;WANG Ying;LIU Xin-yu;ZHANG Xue-mei(Department of Veterinary Medicine,Agriculture College of Yanbian University,Yanji,Jilin,133002,China)

机构地区：[1]延边大学农学院动物医学系,吉林延吉133002

出　　处：《动物医学进展》2018年第12期110-113,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31660727;31260622;31060349);吉林省科技厅重点科技攻关项目(20160204016NY)

摘　　要：通过腹腔注射四氯化碳(CCl4)建立小鼠急性肝损伤模型,研究黄芪粗提物对CCl4诱导的小鼠急性肝损伤的保护作用。设空白组、模型组、阳性组和黄芪粗提物高、中、低剂量组(8、4、2g/kg),连续灌胃给药15d,末次给药后腹腔注射CCl4,分别测定小鼠肝脏指数,血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)及肝脏中甘油三酯(TG)的含量,观察小鼠肝脏病理组织学变化。与模型组比较,黄芪粗提物高、中、低剂量组显著或极显著降低肝脏指数(P<0.01或P<0.05),降低血清中ALT、AST、MDA及肝脏中TG的含量(P<0.01或P<0.05),显著或极显著减轻小鼠肝纤维化的程度(P<0.05,P<0.01)。结果表明,黄芪粗提物对CCl4诱导的小鼠急性肝损伤有保护作用,可为黄芪粗提物在临床上的应用提供理论基础。The model of acute liver injury was established by intraperitoneally injecting CCl4in mice,the protective effect of Astragalus crude extracts on CCl4-induced acute liver injury in mice was investigated.The mice were divided into blank group,model group,positive group and Astragalus crude extracts high,medium and low dose groups(8,4,2g/kg),drugs were administered continuously for15d,the CCl4was intraperitoneally injected after the last administration.The liver index,ALT,AST and MDA in sera,TG in liver tissue were measured.The hepatic histopathological lesions were observed in mice.The results showed that compared with model group,the Astragalus crude extracts high,medium and low dose groups could significantly decrease the liver index of mice(P<0.01or P<0.05),ALT,AST and MDA contents in sera and TG content in liver tissue(P<0.01or P<0.05)and significantly reduced the degree of liver fibrillation in mice.The Astragalus extracts have protective effect on CCl4-induced acute liver injury in mice,which provided a theoretical basis for the clinical application.

关 键 词：黄芪粗提物 四氯化碳 急性肝损伤 小鼠 

分 类 号：S852.31[农业科学—基础兽医学] S853.74[农业科学—兽医学]