2016年辽宁规模化猪场病毒性腹泻流行状况调查分析  

Prevalence Investigation of Swine Viral Diarrhea in Large-scale Pig Farms in Liaoning Area in 2016 and Genetic Evolution Analysis of PEDV S1 Gene

作　　者：关淼[1] 赵晓彤[1] 周晨阳 顾贵波[1] 杨本勇[1] 李井春[1] 赵凤菊[1] 杨洺阳 申贯男[1] 李蓉[1] 王宏燕[1] 张健[1] 梁乔[1] GUAN Miao;ZHAO Xiao-tong;ZHOU Chen-yang;GU Gui-bo;YANG Ben-yong;LI Jing-chun;ZHAO Feng-ju;YANG Ming-yang;SHEN Guan-nan;LI Rong;WANG Hong-yan;ZHANG Jian;LIANG Qiao(Liaoning Center For Provincial Animal Epidemic Disease Control and Prevention,Liaoning Province Veterinary Medicine Institute,Liaoning,Shenyang,110164,China)

机构地区：[1]辽宁省动物疫病预防控制中心,辽宁省动物医学研究院,辽宁沈阳110164

出　　处：《动物医学进展》2018年第12期204-210,共7页Progress In Veterinary Medicine

基　　金：农业攻关及产业化项目(2015202013).

摘　　要：为了解辽宁地区规模化养猪场PEDV、TGEV、PRV-A的流行特征以及PEDV S1基因的遗传演化情况,2016年从辽宁地区14个地市采集700份仔猪粪便样品,通过问卷调查及荧光定量RT-PCR方法对这3种引起猪腹泻的病毒进行流行病学调查。使用特异性引物对PEDV S1基因进行RT-PCR扩增并测序,应用DNA Star软件对该基因序列进行比对和遗传演化分析。结果显示,问卷调查中发生腹泻的场阳性率为58.6%,哺乳仔猪更易发病,发病率为12.2%,死亡率为4.5%。实验室检测结果显示,腹泻的发生主要集中在冬春交接的第一季度,PRV-A样品阳性率(41.3%,31/75)高于PEDV样品阳性率为(36%,27/75),未检出TGEV。遗传演化分析显示12株2016年PEDV辽宁分离株之间的同源性为92.6%～100%,主要属于第3亚群。以上结果表明,在辽宁地区引起猪腹泻的病毒主要为PEDV和PRV-A,且PRV-A更为流行。PEDV在辽宁地区存在不同分支的病毒株流行,且在发展过程中核酸发生了一定的变化。In order to understand the epidemiological characteristics of PEDV,TGEV,PRV-A and the genetic evolution of PEDV S1gene in large-scale pig farms in Liaoning,700fecal samples of piglets were collected from14cities of Liaoning in2016to investigate the epidemiology of these three viruses causing diarrhea in pigs with questionnaire investigation and fluorescent RT-PCR.S1gene of PEDV was amplified and sequenced using RT-PCR with specific primers,and the sequence alignments as well as the genetic variation analysis were conducted by DNAstar.The result showed that the positive rate of farms with diarrhea in the questionnaire was58.6%,and the suckling piglets were more susceptible to infection,with the morbidity at12.2%and the mortality at4.5%.Laboratory tests found that diarrhea mainly happened in the first quarter of a year,between winter and spring,and the positive rate of PRV-A containing samples was41.3%(31/75),higher than that of PEDV positive samples at36%(27/75),while no TGEV was detected.Genetic evolution analysis suggested that the homology of12PEDV isolates from Liaoning in2016was between92.6%-100%,mainly classified to the third group.These results indicated that the major viruses causing swine diarrhea in Liaoning were PEDV and PRV-A,while PRV-A was more prevalent.Various PEDV strains classified to different branches were prevalent in Liaoning area,while their nucleic acids have undergone certain changes during the development.

关 键 词：猪流行性腹泻 猪传染性胃肠炎 猪轮状病毒 遗传演化分析 

分 类 号：S852.65[农业科学—基础兽医学]