松材线虫侵染下马尾松针叶miRNA和mRNA的关联表达  

Expression Correlation Between miRNA and mRNA From Needle Leaves of Pinus massoniana with Bursaphelenchus xylophilus Infestation

作　　者：谢婉凤[1] 梁光红[2] XIE Wan-feng;LIANG Guang-hong(Jinshan College,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Forestry College,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学金山学院,福建福州350002 [2]福建农林大学林学院,福建福州350002

出　　处：《林业科学研究》2018年第6期7-14,共8页Forest Research

基　　金："十三五"国家重点研发计划项目(2017YFD0600105);国家自然科学基金项目(31870641);福建省教育厅中青年教师教育科研基金(JAT170882)

摘　　要：[目的]微小RNA(microRNA,miRNA)具有靶向沉默信使RNA(mRNA)表达的功能,是基因表达的负调控因子;研究并明确马尾松在遭受松材线虫侵染下是否存类似的调控模式,对于未来探析寄主植物对病原侵染胁迫下的应激机制及获得调控马尾松抗松材线虫病的miRNA及其靶标mRNA都具有重要意义。[方法]以前期高通量测序获得的松材线虫侵染1、2、3 d的马尾松针叶mRNA和miRNA表达谱为研究对象,采用STEM软件分别分析mRNA和miRNA的表达变化模式,并运用斯皮尔曼等级相关法研究松材线虫侵染下的马尾松针叶中miRNA与mRNA的表达关联情况。[结果]松材线虫侵染下的马尾松针叶中的miRNA呈2种显著的表达变化模式,mRNA呈8种显著的表达变化模式,且15个miRNA与其12个靶标mRNA的表达变化模式相反,符合miRNA对靶标mRNA的负调控特点,这些靶标mRNA编码具有识别病原作用的ACRE、CC-NBS-LRR基因等。[结论]松材线虫侵染下的马尾松针叶中miRNA及mRNA均呈多种表达变化模式,且部分miRNA与靶标mRNA的表达变化模式相反,推测其中部分miRNA可能是病原识别基因的负调控因子。[Objective]MicroRNA(miRNA)has the function of silence target mRNA expression,which is considered as a negative role in the regulation of gene expression.This study aims to reveal the potential expression correlation between miRNA and mRNA from needle leaves of Pinus massoniana with Bursaphelenchus xylophilus infestation,which helps to further indicate the mechanism of host plant in response to the pathogen infestation,and to obtain the particular miRNA and the target mRNA that involved in the regulation of masson pine resistance to pine wilt disease.[Method]Expression pools of miRNA and mRNA from P.massoniana with B.xylophilus infestation for 1,2,and 3 d,which were generated using RNA-seq in our previous studies,were used as materials.The different expression profiles of miRNA or mRNA from P.massoniana with B.xylophilus infestation for different days were figured using STEM(Short Time-series Expression Miner)software,and method of Spearman Rank Correlation was employed to comparatively analyze the miRNA and mRNA expression profiles of P.massoniana.[Result]Two significant expression profiles were generated from the miRNA pools of P.massoniana with B.xylophilus infestation for 1,2,and 3 d,whilst 8 significant profiles were generated from that of mRNA pools.Fifteen miRNAs were considered as candidates that obviously represented correlation with twelve mRNAs from the expression pools.The reverse expression profiles between the mRNA and miRNA validate the bio-function of transcriptional silence from miRNA to the target mRNA.The miRNA-targeted mRNA encodes ACRE,CC-NBS-LRR resistance-like gene,etc.These genes play roles in plant pathogen recognition.[Conclusion]It was clear that the infestation of B.xylophilus resulted in various expression profiles of miRNA and mRNA from the P.massoniana,and numbers of miRNA presented a reverse expression pattern in comparison with target mRNA.Among these miRNAs,several of them were speculated that function in silenced expression of genes involved with pathogen recognition.

关 键 词：马尾松 松材线虫 微小RNA 信使RNA 关联表达 

分 类 号：S763.3[农业科学—森林保护学]